The PhD Finger Protein VRN5 Functions in the Epigenetic Silencing of Arabidopsis FLC

Greb, Thomas, Mylne, Joshua S., Crevillen, Pedro, Geraldo, Nuno, An, Hailong, Gendall, Anthony R. and Dean, Caroline (2007) The PhD Finger Protein VRN5 Functions in the Epigenetic Silencing of Arabidopsis FLC. Current Biology, 17 1: 73-78. doi:10.1016/j.cub.2006.11.052

Author Greb, Thomas
Mylne, Joshua S.
Crevillen, Pedro
Geraldo, Nuno
An, Hailong
Gendall, Anthony R.
Dean, Caroline
Title The PhD Finger Protein VRN5 Functions in the Epigenetic Silencing of Arabidopsis FLC
Journal name Current Biology   Check publisher's open access policy
ISSN 0960-9822
Publication date 2007-01-09
Sub-type Article (original research)
DOI 10.1016/j.cub.2006.11.052
Volume 17
Issue 1
Start page 73
End page 78
Total pages 6
Place of publication Cambridge, Mass
Publisher Cell Press
Collection year 2006
Language eng
Subject C1
270499 Botany not elsewhere classified
780105 Biological sciences
Abstract Vernalization, the acceleration of flowering by the prolonged cold of winter, ensures that plants flower in favorable spring conditions. During vernalization in Arabidopsis, cold temperatures repress FLOWERING LOCUS C (FLC) expression [1,2] in a mechanism involving VERNALIZATION INSENSITIVE 3 (VIN3) [3], and this repression is epigenetically maintained by a Polycomb-like chromatin regulation involving VERNALIZATION 2 (VRN2), a Su(z)12 homolog, VERNALIZATION 1 (VRN1), and LIKE-HETEROCHROMATIN PROTEIN 1 [4,5,6,7,8]. In order to further elaborate how cold repression triggers epigenetic silencing, we have targeted mutations that result in FLC misexpression both at the end of the prolonged cold and after subsequent development. This identified VERNALIZATION 5 (VRN5), a PHD finger protein and homolog of VIN3. Our results suggest that during the prolonged cold, VRN5 and VIN3 forma heterodimer necessary for establishing the vernalization-induced chromatin modifications, histone deacetylation, and H3 lysine 27 trimethylation required for the epigenetic silencing of FLC. Double mutant and FLC misexpression analyses reveal additional VRN5 functions, both FLC-dependent and -independent, and indicate a spatial complexity to FLC epigenetic silencing with VRN5 acting as a common component in multiple pathways.
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Created: Wed, 15 Aug 2007, 10:53:46 EST