Post-translational modification accounts for the presence of varied forms of nerve growth factor in Australian elapid snake venoms

Earl, Stephen, Birrell, Geoffrey, Wallis, Tristan, St Pierre, Liam, Masci, Paul, de Jersey, John, Gorman, Jeffrey and Lavin, Martin (2006) Post-translational modification accounts for the presence of varied forms of nerve growth factor in Australian elapid snake venoms. Proteomics, 6 24: 6554-6565. doi:10.1002/pmic.200600263


Author Earl, Stephen
Birrell, Geoffrey
Wallis, Tristan
St Pierre, Liam
Masci, Paul
de Jersey, John
Gorman, Jeffrey
Lavin, Martin
Title Post-translational modification accounts for the presence of varied forms of nerve growth factor in Australian elapid snake venoms
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9853
Publication date 2006-11-15
Sub-type Article (original research)
DOI 10.1002/pmic.200600263
Volume 6
Issue 24
Start page 6554
End page 6565
Total pages 12
Place of publication Weinheim, Germany
Publisher Wiley
Collection year 2006
Language eng
Subject 1103 Clinical Sciences
Abstract The Australian elapid snakes are amongst the most venomous snakes in the world, but much less is known about the overall venom composition in comparison to Asian and American snakes. We have used a combined approach of cDNA cloning and 2-DE with MS to identify nerve growth factor (NGF) in venoms of the Australian elapid snakes and demonstrate its neurite outgrowth activity While a single 730 nucleotide ORF, coding for a 243 amino acid precursor protein was detected in all snakes, use of 2-DE identified NGF proteins with considerable variation in molecular size within and between the different snakes. The variation in size can be explained at least in part by Winked glycosylation. it is possible that these modifications alter the stability, is necessary to activity and other characteristics of the snake NGFs. Further characterisation delineate the function of the individual NGF isoforms.
Keyword 2-DE
Glycosylation sites
Australian elapid
Venom
Nerve growth factor
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 20:42:01 EST