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A blank slate? Layer-by-layer deposition of hyaluronic acid and chitosan onto various surfaces
Croll, Tristan I., OConnor, Andrea J., Stevens, Geoffrey W. and Cooper-White, Justin J. (2006) A blank slate? Layer-by-layer deposition of hyaluronic acid and chitosan onto various surfaces. Biomacromolecules, 7 5: 1610-1622.
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| Author(s) |
Croll, Tristan I.
OConnor, Andrea J.
Stevens, Geoffrey W.
Cooper-White, Justin J.
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| Title |
A blank slate? Layer-by-layer deposition of hyaluronic acid and chitosan onto various surfaces
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| Journal name |
Biomacromolecules
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| Publication date |
2006
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| Volume number |
7
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| Issue number |
5
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| ISSN |
1525-7797
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| Start page |
1610
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| End page |
1622
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| Total pages |
13
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| Editor(s) |
Ann-Christine Albertsson
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| Place of publication |
Washington
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| Publisher |
American Chemical Society
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| Collection year |
2006
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| Language |
eng
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| Subject |
C1
290600 Chemical Engineering
670705 Plastic products (incl. construction materials)
CX
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| Abstract |
Although poly(alpha-hydroxy esters), especially the PLGA family of lactic acid/glycolic acid copolymers, have many properties which make them promising materials for tissue engineering, the inherent chemistry of surfaces made from these particular polymers is problematic. In vivo, they promote a strong foreign-body response as a result of nonspecific adsorption and denaturation of serum proteins, which generally results in the formation of a nonfunctional fibrous capsule. Surface modification post-production of the scaffolds is an often-utilized approach to solving this problem, conceptually allowing the formation of a scaffold with mechanical properties defined by the bulk material and molecular-level interactions defined by the modified surface properties. A promising concept is the so-called blank slate: essentially a surface that is rendered resistant to nonspecific protein adsorption but can be readily activated to covalently bind bio-functional molecules such as extracellular matrix proteins, growth factors or polysaccharides. This study focuses on the use of the quartz crystal microbalance (QCM) to follow the layer-by-layer (LbL) electrostatic deposition of high molecular weight hyaluronic acid and chitosan onto PLGA surfaces rendered positively charged by aminolysis, to form a robust, protein-resistant coating. We further show that this surface may be further functionalized via the covalent attachment of collagen IV, which may then be used as a template for the self-assembly of basement membrane components from dilute Matrigel. The response of NIH-3T3 fibroblasts to these surfaces was also followed and shown to closely parallel the results observed in the QCM.
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| Keyword(s) |
Quartz-crystal Microbalance
In-vitro
Polymeric Scaffolds
Poly(lactic Acid)
Cell Interactions
Entrapment
Aminolysis
Films
Biomaterials
Hydrolysis
Biochemistry & Molecular Biology
Chemistry, Organic
Polymer Science
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