A blank slate? Layer-by-layer deposition of hyaluronic acid and chitosan onto various surfaces

Croll, Tristan I., OConnor, Andrea J., Stevens, Geoffrey W. and Cooper-White, Justin J. (2006) A blank slate? Layer-by-layer deposition of hyaluronic acid and chitosan onto various surfaces. Biomacromolecules, 7 5: 1610-1622. doi:10.1021/bm060044l

Author Croll, Tristan I.
OConnor, Andrea J.
Stevens, Geoffrey W.
Cooper-White, Justin J.
Title A blank slate? Layer-by-layer deposition of hyaluronic acid and chitosan onto various surfaces
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
Publication date 2006
Sub-type Article (original research)
DOI 10.1021/bm060044l
Volume 7
Issue 5
Start page 1610
End page 1622
Total pages 13
Editor Ann-Christine Albertsson
Place of publication Washington
Publisher American Chemical Society
Collection year 2006
Language eng
Subject C1
290600 Chemical Engineering
670705 Plastic products (incl. construction materials)
Abstract Although poly(alpha-hydroxy esters), especially the PLGA family of lactic acid/glycolic acid copolymers, have many properties which make them promising materials for tissue engineering, the inherent chemistry of surfaces made from these particular polymers is problematic. In vivo, they promote a strong foreign-body response as a result of nonspecific adsorption and denaturation of serum proteins, which generally results in the formation of a nonfunctional fibrous capsule. Surface modification post-production of the scaffolds is an often-utilized approach to solving this problem, conceptually allowing the formation of a scaffold with mechanical properties defined by the bulk material and molecular-level interactions defined by the modified surface properties. A promising concept is the so-called blank slate: essentially a surface that is rendered resistant to nonspecific protein adsorption but can be readily activated to covalently bind bio-functional molecules such as extracellular matrix proteins, growth factors or polysaccharides. This study focuses on the use of the quartz crystal microbalance (QCM) to follow the layer-by-layer (LbL) electrostatic deposition of high molecular weight hyaluronic acid and chitosan onto PLGA surfaces rendered positively charged by aminolysis, to form a robust, protein-resistant coating. We further show that this surface may be further functionalized via the covalent attachment of collagen IV, which may then be used as a template for the self-assembly of basement membrane components from dilute Matrigel. The response of NIH-3T3 fibroblasts to these surfaces was also followed and shown to closely parallel the results observed in the QCM.
Keyword Quartz-crystal Microbalance
Polymeric Scaffolds
Poly(lactic Acid)
Cell Interactions
Biochemistry & Molecular Biology
Chemistry, Organic
Polymer Science
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 10:39:21 EST