Use of optical density as a measure of Claviceps africana conidial suspension concentration

Herde, D. J., Ryley, M. J., Foster, S. D., Galea, V. J., Henzell, R. G. and Jordan, D. R. (2006) Use of optical density as a measure of Claviceps africana conidial suspension concentration. Australasian Plant Pathology, 35 1: 77-80. doi:10.1071/AP05093


Author Herde, D. J.
Ryley, M. J.
Foster, S. D.
Galea, V. J.
Henzell, R. G.
Jordan, D. R.
Title Use of optical density as a measure of Claviceps africana conidial suspension concentration
Journal name Australasian Plant Pathology   Check publisher's open access policy
ISSN 0815-3191
1448-6032
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1071/AP05093
Volume 35
Issue 1
Start page 77
End page 80
Total pages 4
Editor Chris Anderson
Place of publication Australia
Publisher C.S.I.R.O. Publishing
Collection year 2006
Language eng
Subject C1
300204 Plant Protection (Pests, Diseases and Weeds)
620104 Other cereals
Abstract Sorghum ergot, caused by Claviceps africana, has remained a major disease problem in Australia since it was first recorded in 1996, and is the focus of a range of biological and integrated management research. Artificial inoculation using conidial suspensions is an important tool in this research. Ergot infection is greatly influenced by environmental factors, so it is important to reduce controllable sources of variation such as inoculum concentration. The use of optical density was tested as a method of quantifying conidial suspensions of C. africana, as an alternative to haemocytometer counts. This method was found to be accurate and time efficient, with possible applications in other disease systems.
Keyword Colorimeter
Inoculum Conceentration
Light Transmittance
Sorghum Bicolor
Plant Sciences
Inoculum Concentration
Sorghum
Ergot
Disease
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 20:38:43 EST