Neural Stem cell Isolation and Characterization

Rietze, R. L. and Reynolds, B. A. (2006) Neural Stem cell Isolation and Characterization. Methods in Enzymology: Adult Stem Cells, 419 3-23. doi:10.1016/S0076-6879(06)19001-1

Author Rietze, R. L.
Reynolds, B. A.
Title Neural Stem cell Isolation and Characterization
Journal name Methods in Enzymology: Adult Stem Cells   Check publisher's open access policy
ISSN 0076-6879
ISBN 9780123736505
Publication date 2006
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/S0076-6879(06)19001-1
Volume 419
Start page 3
End page 23
Total pages 21
Editor I. Klimanskaya
R. Lanza
Place of publication New York
Publisher Academic Press
Collection year 2006
Language eng
Subject C1
320702 Central Nervous System
730104 Nervous system and disorders
Abstract Throughout the process of development and continuing into adulthood, stem cells function as a reservoir of undifferentiated cell types, whose role is to underpin cell genesis in a variety of tissues and organs. In the adult, they play an essential homeostatic role by replacing differentiated tissue cells "worn off" by physiological turnover or lost to injury or disease. As such, the discovery of such cells in the adult mammalian central nervous system (CNS), an organ traditionally thought to have little or no regenerative capacity, was most unexpected. Nonetheless, by employing a novel serum-free culture system termed the neurosphere assay, Reynolds and Weiss demonstrated the presence of neural stem cells in both the adult (Reynolds and Weiss, 1992) and embryonic mouse brain (Reynolds et al., 1992). Here we describe how to generate, serially passage, and differentiate neurospheres derived from both the developing and adult brain, and provide more technical details that will enable one to achieve reproducible cultures, which can be passaged over an extended period of time.
Q-Index Code C1

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
2007 Higher Education Research Data Collection
Queensland Brain Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 126 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 147 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 10:33:35 EST