The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b

Simpson, Fiona, Lammerts van Bueren, Kelly L., Butterfield, Natalie, Bennetts, Jennifer S., Bowles, Josephine, Adolphe, Christelle, Simms, Lisa A., Young, Joanne, Walsh, Michael D., Leggett, Barbara, Fowles, Lindsay F. and Wicking, Carol (2006) The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b. Experimental Cell Research, 312 1: 73-85. doi:10.1016/j.yexcr.2005.09.020


Author Simpson, Fiona
Lammerts van Bueren, Kelly L.
Butterfield, Natalie
Bennetts, Jennifer S.
Bowles, Josephine
Adolphe, Christelle
Simms, Lisa A.
Young, Joanne
Walsh, Michael D.
Leggett, Barbara
Fowles, Lindsay F.
Wicking, Carol
Title The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b
Formatted title
The PCNA-associated factor KIAA0101/p15PAF binds the potential tumor suppressor product p33ING1b

Journal name Experimental Cell Research   Check publisher's open access policy
ISSN 0014-4827
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1016/j.yexcr.2005.09.020
Volume 312
Issue 1
Start page 73
End page 85
Total pages 13
Editor U. Lendahl
Place of publication San Diego
Publisher Elsevier
Collection year 2006
Language eng
Subject C1
270199 Biochemistry and Cell Biology not elsewhere classified
780105 Biological sciences
Abstract The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera raised against a p15(PAF) fusion protein, we have shown that in a range of mammalian tumor and non-tumor cell lines the endogenous p15(PAF) protein localises to the nucleus and the mitochondria. Under normal conditions no co-localisation with PCNA could be detected, however following exposure to UV it was possible to co-immunoprecipitate p15(PAF) and PCNA from a number of cell lines, suggesting a UV-enhanced association of the two proteins. Overexpression of p15(PAF) in mammalian cells was also found to protect cells from UV-induced cell death. Based on similarities between the behaviour of p15(PAF) and the potential tumor suppressor product p33ING1b, we have further shown that these two proteins interact in the same complex in cell cultures. This suggests that p15(PAF) forms part of a larger protein complex potentially involved in the regulation of DNA repair, apoptosis and cell cycle progression. (c) 2005 Elsevier Inc. All rights reserved.
Keyword KIAA0101
colorectal tumor
PCNA
p33ING1b
UV irradiation
OEATC-1
Cell Lung-cancer
Colorectal-cancer
Dna-repair
Gene Ing1
Expression
Inhibitor
Proteins
Complex
Replication
Acetylation
Q-Index Code C1

 
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Created: Thu, 16 Aug 2007, 10:07:23 EST