Specific detection of enterovirus 71 directly from clinical specimens using real-time RT-PCR hybridization probe assay

Tan, E. L., Chow, Tak Kwong, Kumarasinghe, G., Lin, T. Tze Pin, Mackay, I. M., Sloots, T. P. and Poh, C. L. (2006) Specific detection of enterovirus 71 directly from clinical specimens using real-time RT-PCR hybridization probe assay. Molecular and Cellular Probes, 20 2: 135-140. doi:10.1016/j.mcp.2005.11.003


Author Tan, E. L.
Chow, Tak Kwong
Kumarasinghe, G.
Lin, T. Tze Pin
Mackay, I. M.
Sloots, T. P.
Poh, C. L.
Title Specific detection of enterovirus 71 directly from clinical specimens using real-time RT-PCR hybridization probe assay
Journal name Molecular and Cellular Probes   Check publisher's open access policy
ISSN 0890-8508
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1016/j.mcp.2005.11.003
Volume 20
Issue 2
Start page 135
End page 140
Total pages 6
Editor Alan F Scott
Alvin Fox
Editors in Chief
Place of publication England
Publisher Academic Press
Collection year 2006
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract Enterovirus 71 (EV71) is one of the main causative agents of hand, foot and mouth disease (HFMD) in young children. Infections caused by EV71 could lead to many complications, ranging from brainstem encephalitis to pulmonary oedema, resulting in high mortality. Thus, rapid detection of the virus is required to enable measures to be implemented in preventing widespread transmission. Based on primers and probes targeting at the VP1 region, a real-time reverse-transcriptase polymerase chain reaction (RT-PCR) hybridization probe assay was developed for specific detection of EV71 from clinical specimens. Quantitative analysis showed that the assay was able to detect as low as 5 EV71 viral copies and EV71 was detected from 46 of the 55 clinical specimens obtained from pediatric patients suffering from HFMD during the period from 2000 to 2003 in Singapore. This study showed that the single tube real-time RT-PCR assay developed in this study can be applied as a rapid and sensitive method for specific detection of EV71 directly from clinical specimens. (c) 2005 Elsevier Ltd. All rights reserved.
Keyword Biochemical Research Methods
Hand
Biotechnology & Applied Microbiology
Cell Biology
Rapid detection
Hand, Foot And Mouth Disease (hfmd)
Quantitative Real-time Hybridization Probe Assay
Rapid Detection
Mouth-disease Outbreak
Foot
Hand
Evolution
Epidemic
Infection
Singapore
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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Created: Wed, 15 Aug 2007, 20:23:53 EST