Lineages of acidophilic archaea revealed by community genomic analysis

Baker, Brett J., Tyson, Gene W., Webb, Richard I., Flanagan, Judith, Hugenholtz, Philip, Allen, Eric E. and Banfield, Jillian F. (2006) Lineages of acidophilic archaea revealed by community genomic analysis. Science, 314 5807: 1933-1935. doi:10.1126/science.1132690

Author Baker, Brett J.
Tyson, Gene W.
Webb, Richard I.
Flanagan, Judith
Hugenholtz, Philip
Allen, Eric E.
Banfield, Jillian F.
Title Lineages of acidophilic archaea revealed by community genomic analysis
Journal name Science   Check publisher's open access policy
ISSN 0036-8075
Publication date 2006-12-22
Sub-type Article (original research)
DOI 10.1126/science.1132690
Open Access Status Not Open Access
Volume 314
Issue 5807
Start page 1933
End page 1935
Total pages 3
Place of publication United States
Publisher American Association for the Advancement of Science
Collection year 2006
Language eng
Subject C1
780105 Biological sciences
270300 Microbiology
Abstract Novel, low-abundance microbial species can be easily overlooked in standard polymerase chain reaction (PCR)-based surveys. We used community genomic data obtained without PCR or cultivation to reconstruct DNA fragments bearing unusual 16S ribosomal RNA ( rRNA) and protein-coding genes from organisms belonging to novel archaeal lineages. The organisms are minor components of all biofilms growing in pH 0.5 to 1.5 solutions within the Richmond Mine, California. Probes specific for 16S rRNA showed that the fraction less than 0.45 micrometers in diameter is dominated by these organisms. Transmission electron microscope images revealed that the cells are pleomorphic with unusual folded membrane protrusions and have apparent volumes of < 0.006 cubic micrometer.
Keyword Multidisciplinary Sciences
Acid-mine Drainage
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 10:14:53 EST