Vehicle effects on the in vitro penetration of testosterone through equine skin

Mills, P. C. (2007) Vehicle effects on the in vitro penetration of testosterone through equine skin. Veterinary Research Communications, 31 2: 227-233. doi:10.1007/s11259-006-3446-6


Author Mills, P. C.
Title Vehicle effects on the in vitro penetration of testosterone through equine skin
Formatted title
Vehicle effects on the in vitro penetration of testosterone through equine skin
Journal name Veterinary Research Communications   Check publisher's open access policy
ISSN 0165-7380
Publication date 2007-02
Year available 2006
Sub-type Article (original research)
DOI 10.1007/s11259-006-3446-6
Volume 31
Issue 2
Start page 227
End page 233
Total pages 7
Editor A. van Miert
Place of publication Netherlands
Publisher Springer-Verlag
Collection year 2006
Language eng
Subject C1
300512 Pharmacology
780105 Biological sciences
Abstract The effects of three vehicles, phosphate-buffered saline (PBS), ethanol (50% in PBS w/w) and propylene glycol (50% in PBS w/w) on in vitro transdermal penetration of testosterone was investigated in the horse. Skin was harvested from the thorax of five Thoroughbred horses after euthanasia and stored at -20 degrees C until required. The skin was then defrosted and placed into Franz-type diffusion cells, which were maintained at approximately 32 degrees C by a water bath. Saturated solutions of testosterone, containing trace amounts of radiolabelled [C-14]testosterone, in each vehicle were applied to the outer (stratum corneum) surface of each skin sample and aliquots of receptor fluid were collected at 0, 2, 4, 8, 16, 20, 22 and 24 h and analysed for testosterone by scintillation counting. The maximum flux (J(max)) of testosterone was significantly higher for all sites when testosterone was dissolved in a vehicle containing 50% ethanol or 50% propylene glycol, compared to PBS. In contrast, higher residues of testosterone were found remaining within the skin when PBS was used as a vehicle. This study shows that variability in clinical response to testosterone could be expected with formulation design.
Keyword horse
testosterone
Thoroughbred
topical
transdermal
Percutaneous-absorption
Transfollicular Routes
Hydrocortisone
Permeation
Pharmacokinetics
Steroids
Therapy
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 10:05:51 EST