Assessment of epidermal cell viability by near infrared multi-photon microscopy following ballistic delivery of gold micro-particles

Raju, P. A., McSloy, N., Truong, N. K. and Kendall, M. A. F. (2006) Assessment of epidermal cell viability by near infrared multi-photon microscopy following ballistic delivery of gold micro-particles. Vaccine, 24 21: 4644-4647.


Author Raju, P. A.
McSloy, N.
Truong, N. K.
Kendall, M. A. F.
Title Assessment of epidermal cell viability by near infrared multi-photon microscopy following ballistic delivery of gold micro-particles
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
Publication date 2006
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2005.08.067
Volume 24
Issue 21
Start page 4644
End page 4647
Total pages 4
Editor R. E. Spier
R. Strugnell
Place of publication United Kingdom
Publisher Elsevier Ltd
Collection year 2006
Language eng
Subject C1
291599 Biomedical Engineering not elsewhere classified
730101 Infectious diseases
110799 Immunology not elsewhere classified
Abstract The use of gene guns in ballistically delivering DNA vaccine coated gold micro-particles to skin can potentially damage targeted cells, therefore influencing transfection efficiencies. In this paper, we assess cell death in the viable epidermis by non-invasive near infrared two-photon microscopy following micro-particle bombardment of murine skin. We show that the ballistic delivery of micro-particles to the viable epidermis can result in localised cell death. Furthermore, experimental results show the degree of cell death is dependant on the number of micro-particles delivered per unit of tissue surface area. Micro-particles densities of 0.16 +/- 0.27 (mean +/- S.D.), 1.35 +/- 0.285 and 2.72 +/- 0.47 per 1000 mu m(2) resulted in percent deaths of 3.96 +/- 5.22, 45.91 +/- 10.89, 90.52 +/- 12.28, respectively. These results suggest that optimization of transfection by genes administered with gene guns is - among other effects - a compromise of micro-particle payload and cell death. (c) 2005 Elsevier Ltd. All rights reserved.
Keyword Immunology
Medicine, Research & Experimental
Veterinary Sciences
Biolistics
Dna Vaccine
Multi-photon Microscopy
Stratum-corneum
Dendritic Cells
Induction
Skin
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 10:03:15 EST