Resistance of porcine blood clots to lysis relates to poor activation of porcine plasminogen by tissue plasminogen activator

Flight, SM, Masci, PP, Lavin, MF and Gaffney, PJ (2006) Resistance of porcine blood clots to lysis relates to poor activation of porcine plasminogen by tissue plasminogen activator. Blood Coagulation & Fibrinolysis, 17 5: 417-420. doi:10.1097/01.mbc.0000233374.79593.57


Author Flight, SM
Masci, PP
Lavin, MF
Gaffney, PJ
Title Resistance of porcine blood clots to lysis relates to poor activation of porcine plasminogen by tissue plasminogen activator
Journal name Blood Coagulation & Fibrinolysis   Check publisher's open access policy
ISSN 0957-5235
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1097/01.mbc.0000233374.79593.57
Volume 17
Issue 5
Start page 417
End page 420
Total pages 4
Editor E. L. Saenko
R. Marlar
Place of publication USA
Publisher Lippincott Williams & Wilkins
Collection year 2006
Language eng
Subject C1
321008 Haematology
730103 Blood disorders
Abstract In-vitro experimentation was performed on porcine and human blood to determine their comparative responsiveness to a novel fibrinolytic inhibitor and thereby assess whether the pig is a suitable animal model for subsequent in-vivo testing of this inhibitor. Thromboelastography showed the clots formed from porcine whole blood to be highly resistant to tissue plasminogen activator (t-PA)-catalyzed lysis, and this communication offers the resistance of porcine plasminogen to activation by t-PA as an explanation. Porcine blood containing 100 and 1500 IU/ml added t-PA lysed very slowly, having LY30 values of 1.9 +/- 1.4 and 2.9 +/- 1.9%, respectively. In contrast, the LY30 values for the human clots containing 100 and 1500 IU/ml t-PA were 77.1 +/- 6.3 and 93.3 +/- 1.3%, respectively. Moreover, purified porcine plasminogen was activated very slowly by added t-PA in the presence of both human and porcine fibrin. Activation of plasminogen by the endogenous activators, as measured by the euglobulin clot lysis time, was greatly prolonged for the pig (22 +/- 3 h) compared with the human (3.5 +/- 1.5 h). These results suggest caution in using the pig as an experimental model when studying the effects of various agents on fibrinolysis.
Keyword Hematology
Fibrinolysis
Plasminogen Activation
Porcine
Thromboelastography
Affinity Chromatography
Urokinase
Peptide
Model
Mechanism
Plaque
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2007 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 19:59:04 EST