Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis

Smith, Lucas, Rose, Barbara, Tingpej, Pholawat, Zhu, Hua, Conibear, Tim, Manos, Jim, Bye, Peter, Elkins, Mark, Willcox, Mark, Bell, Scoot, Wainwright, Claire and Harbour, Colin (2006) Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis. Journal of Medical Microbiology, 55 12: 1641-1644. doi:10.1099/jmm.0.46845-0


Author Smith, Lucas
Rose, Barbara
Tingpej, Pholawat
Zhu, Hua
Conibear, Tim
Manos, Jim
Bye, Peter
Elkins, Mark
Willcox, Mark
Bell, Scoot
Wainwright, Claire
Harbour, Colin
Title Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis
Journal name Journal of Medical Microbiology   Check publisher's open access policy
ISSN 0022-2615
1473-5644
Publication date 2006-12
Sub-type Article (original research)
DOI 10.1099/jmm.0.46845-0
Volume 55
Issue 12
Start page 1641
End page 1644
Total pages 4
Editor I. R. Poxton
Place of publication Reading, United Kingdom
Publisher Society for General Microbiology
Collection year 2006
Language eng
Subject 320401 Medical Bacteriology
730101 Infectious diseases
Abstract Protease IV is important in the pathogenesis of Pseudomonas aeruginosa-induced microbial keratitis, but little is known of its role in cystic fibrosis (CF) lung infection. In this study protease IV production was examined in 43 P. aeruginosa isolates (24 non-clonal and 19 clonal) from the lungs of chronically infected adult patients attending the Royal Prince Alfred Hospital CF Clinic, Sydney, Australia. Overall, 32/43 (74 %) isolates were positive for protease IV protein by Western blotting and 22/43 (51 %) had evidence of active protease IV on gelatin zymography. Clonal strains were 1.6 times more likely than non-clonal strains to produce protease IV [18/19 (95 %) versus 14/24 (58 %), RR=1.6, CI 1.1–2.3, P=0.007] and 3 times more likely to secrete the protein [16/19 (84 %) versus 6/24 (25 %), RR=3.4, CI 1.6–6.9, P<0.001]. Nine of the ten strains negative by both Western blotting and zymography were non-clonal, and all but one of these was positive for the protease IV gene. There was a marked strain-to-strain variation in the amount of protease IV produced. Secretion of protease IV by clonal strains may enhance their infectivity and ability to adapt to the changing CF lung environment. Overall the findings suggest that protease IV plays an important role in the pathogenesis of P. aeruginosa infection in the CF lung.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2007 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 09:52:07 EST