Agrobacterium rhizogenes transformation of the Phaseolus spp.: A tool for functional genomics

Estrada-Navarrete, Georgina, Alvarado-Affantranger, Xochitl, Olivares, Juan-Elías, Diaz-Camino, Claudia, Santana, Olivia, Murillo, Enrique, Guillen, Gabriel, Sanchez-Guevara, Nayeli, Acosta, Jorge, Quinto, Carmen, Li, Dongxue, Gresshoff, Peter M. and Sanchez, Federico (2006) Agrobacterium rhizogenes transformation of the Phaseolus spp.: A tool for functional genomics. Molecular Plant-microbe Interactions, 19 12: 1385-1393.


Author Estrada-Navarrete, Georgina
Alvarado-Affantranger, Xochitl
Olivares, Juan-Elías
Diaz-Camino, Claudia
Santana, Olivia
Murillo, Enrique
Guillen, Gabriel
Sanchez-Guevara, Nayeli
Acosta, Jorge
Quinto, Carmen
Li, Dongxue
Gresshoff, Peter M.
Sanchez, Federico
Title Agrobacterium rhizogenes transformation of the Phaseolus spp.: A tool for functional genomics
Formatted title Agrobacterium rhizogenes transformation of the Phaseolus spp.: A tool for functional genomics
Journal name Molecular Plant-microbe Interactions   Check publisher's open access policy
ISSN 0894-0282
Publication date 2006
Sub-type Article (original research)
DOI 10.1094/MPMI-19-1385
Volume 19
Issue 12
Start page 1385
End page 1393
Total pages 9
Editor Gary Stacey
Place of publication St Paul
Publisher American Phytopathological Society
Collection year 2006
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
620108 Grain legumes
Abstract A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: R coccineus, P lunatus, and P acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and beta-glucuronidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root-microbe interactions.
Keyword Common Bean
T-dna
Transgenic Nodules
Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Plant Sciences
Transgenic Root-nodules
Mediated Transformation
Medicago-truncatula
Lotus-japonicus
Hairy Roots
Vulgaris L
Gene
Plant
Legumes
System
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 09:41:14 EST