Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix

Vashi, Aditya V., Abberton, Keren M., Thomas, Gregory P., Morrison, Wayne A., O'Connor, Andrea J., Cooper-White, Justin J. and Thompson, Erik W. (2006) Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix. Tissue Engineering, 12 11: 3035-3043.


Author Vashi, Aditya V.
Abberton, Keren M.
Thomas, Gregory P.
Morrison, Wayne A.
O'Connor, Andrea J.
Cooper-White, Justin J.
Thompson, Erik W.
Title Adipose tissue engineering based on the controlled release of fibroblast growth factor-2 in a collagen matrix
Journal name Tissue Engineering   Check publisher's open access policy
ISSN 1076-3279
Publication date 2006
Sub-type Article (original research)
DOI 10.1089/ten.2006.12.3035
Volume 12
Issue 11
Start page 3035
End page 3043
Total pages 9
Editor Dr. Anthonios G. Mikos
Dr. Peter C Johnson (Editor-in-Chief)
Place of publication New Rochelle
Publisher Mary Ann Liebert Inc
Collection year 2006
Language eng
Subject C1
290600 Chemical Engineering
670705 Plastic products (incl. construction materials)
Abstract Adipose tissue forms when basement membrane extract ( Matrigel (TM)) and fibroblast growth factor-2 (FGF-2) are added to our mouse tissue engineering chamber model. A mouse tumor extract, Matrigel is unsuitable for human clinical application, and finding an alternative to Matrigel is essential. In this study we generated adipose tissue in the chamber model without using Matrigel by controlled release of FGF-2 in a type I collagen matrix. FGF-2 was impregnated into biodegradable gelatin microspheres for its slow release. The chambers were filled with these microspheres suspended in 60 mu L collagen gel. Injection of collagen containing free FGF-2 or collagen containing gelatin microspheres with buffer alone served as controls. When chambers were harvested 6 weeks after implantation, the volume and weight of the tissue obtained were higher in the group that received collagen and FGF-2 impregnated microspheres than in controls. Histologic analysis of tissue constructs showed the formation of de novo adipose tissue accompanied by angiogenesis. In contrast, control groups did not show extensive adipose tissue formation. In conclusion, this study has shown that de novo formation of adipose tissue can be achieved through controlled release of FGF-2 in collagen type I in the absence of Matrigel.
Keyword MESENCHYMAL STEM-CELLS
DE-NOVO ADIPOGENESIS
GELATIN MICROSPHERES
EXTRACELLULAR-MATRIX
IN-VITRO
DIFFERENTIATION
SCAFFOLD
FAT
REGENERATION
PREADIPOCYTES
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 09:41:07 EST