Vaccination of dogs against Echinococcus granulosus, the cause of cystic hydatid disease in humans

Zhang, Wenbao, Zhang, Zhuangzhi, Shi, Baoxin, Li, Jun, You, Hong, Tulson, Gunlor, Dang, Xinsheng, Song, Yingchun, Yimiti, Turhong, Wang, Jincheng, Jones, Malcolm K. and McManus, Donald P. (2006) Vaccination of dogs against Echinococcus granulosus, the cause of cystic hydatid disease in humans. Journal of Infectious Diseases, 194 7: 966-974. doi:10.1086/506622


Author Zhang, Wenbao
Zhang, Zhuangzhi
Shi, Baoxin
Li, Jun
You, Hong
Tulson, Gunlor
Dang, Xinsheng
Song, Yingchun
Yimiti, Turhong
Wang, Jincheng
Jones, Malcolm K.
McManus, Donald P.
Title Vaccination of dogs against Echinococcus granulosus, the cause of cystic hydatid disease in humans
Journal name Journal of Infectious Diseases   Check publisher's open access policy
ISSN 0022-1899
1537-6613
Publication date 2006-10-01
Sub-type Article (original research)
DOI 10.1086/506622
Volume 194
Issue 7
Start page 966
End page 974
Total pages 9
Editor Martin Hirsch
Place of publication Chicago, U.S.A.
Publisher University Chicago Press
Collection year 2006
Language eng
Subject CX
Formatted abstract
Dogs are pivotal in Echinococcus granulosus transmission to humans, and dog vaccination provides a very practical and cost‐effective prevention strategy. We vaccinated dogs with soluble native proteins isolated from protoscoleces of E. granulosus and induced significant suppression of worm growth and egg production. Accordingly, we tested for vaccine efficacy using recombinant proteins derived from a developmentally regulated gene family (egM) specifically expressed in mature adult E. granulosus worms. Three egM genes—egM4, egM9, and egM123—were subcloned into an expression vector that expressed the molecules as soluble glutathione S‐transferase (GST) fusion proteins in Escherichia coli. The 3 fusion proteins were purified for dog vaccination trials (3 doses of 80 μg/protein/dog) in which the dogs were challenged and then necropsied 45 days after infection. Compared with worms in the control dogs that received GST, the 3 recombinant proteins induced a very high level of protection (97%–100%) in terms of suppression of worm growth and, especially, of egg development and embryogenesis. We have thus shown that vaccination of the dog host against E. granulosus is feasible when recombinant proteins are used. Because the egg stage is crucial in the echinococcal life cycle, successful suppression of egg development by vaccination would halt transmission to intermediate hosts, thereby effecting long‐term control.
Keyword Canine echinococcosis
Echinococcus granulosus
Helminth Proteins
Oncosphere
Antigens
Immunity
Immunisation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Wed, 15 Aug 2007, 19:27:40 EST