Ultrastructure, osmotic tolerance, glycerol toxicity and cryopreservation of caput and cauda epididymidal kangaroo spermatozoa

McClean, Rhett, MacCallum, Catriona, Blyde, David, Holt, William V. and Johnston, Stephen D. (2006) Ultrastructure, osmotic tolerance, glycerol toxicity and cryopreservation of caput and cauda epididymidal kangaroo spermatozoa. Reproduction Fertility And Development, 18 4: 469-476. doi:10.1071/RD05148


Author McClean, Rhett
MacCallum, Catriona
Blyde, David
Holt, William V.
Johnston, Stephen D.
Title Ultrastructure, osmotic tolerance, glycerol toxicity and cryopreservation of caput and cauda epididymidal kangaroo spermatozoa
Journal name Reproduction Fertility And Development   Check publisher's open access policy
ISSN 1031-3613
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1071/RD05148
Volume 18
Issue 4
Start page 469
End page 476
Total pages 8
Editor Sharon Mortimer
Place of publication Collingwood
Publisher Csiro Publishing
Collection year 2006
Language eng
Subject C1
270602 Animal Physiology - Cell
780105 Biological sciences
Abstract The aim of the present study was to compare cryopreservation, osmotic tolerance and glycerol toxicity between mature and immature epididymal kangaroo spermatozoa to investigate whether the lack of cryopreservation success of cauda epididymidal spermatozoa may be related to the increased complexity of the sperm ultrastructure acquired during epididymal transit. Caput and cauda epididymidal spermatozoa were recovered from red-necked wallabies (RNW; Macropus rufogriseus) and eastern grey kangaroos (EGK; M. giganteus). In Experiment 1, caput and cauda epididymidal spermatozoa were frozen and thawed using a standard cryopreservation procedure in Triscitrate buffer with or without 20% glycerol. Although cryopreservation of caput epididymidal spermatozoa resulted in a significant increase in sperm plasma membrane damage, they were more tolerant of the procedure than spermatozoa recovered from the cauda epididymidis (P< 0.05). In Experiment 2, caput and cauda epididymidal EGK spermatozoa were diluted into phosphate-buffered saline media of varying osmolarity and their osmotic tolerance determined. Plasma membranes of caput epididymidal spermatozoa were more tolerant of hypo-osmotic media than were cauda epididymidal spermatozoa ( P< 0.05). In Experiment 3, caput and cauda epididymidal RNW spermatozoa were incubated in Tris-citrate buffer with and without 20% glycerol at 35 and 4 degrees C to examine the cytotoxic effects of glycerol. At both temperatures, caput epididymidal spermatozoa showed less plasma membrane damage compared with cauda epididymidal spermatozoa when exposed to 20% glycerol ( P< 0.05). These experiments clearly indicate that epididymal maturation of kangaroo spermatozoa results in a decreased ability to withstand the physiological stresses associated with cryopreservation.
Keyword Epididymis
Osmolality
Sperm Maturation
Developmental Biology
Reproductive Biology
Zoology
Koala Phascolarctos-cinereus
Trichosurus-vulpecula
Macropus-eugenii
Brushtail Possum
Tammar Wallaby
Cold Shock
Semen
Preservation
Surface
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 19:26:59 EST