The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate)

Sellars, M. J., Coman, F. E., Degnan, B. M. and Preston, N. P. (2006) The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate). Journal of Shellfish Research, 25 2: 631-637. doi:10.2983/0730-8000(2006)25[631:TEOHCA]2.0.CO;2

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Author Sellars, M. J.
Coman, F. E.
Degnan, B. M.
Preston, N. P.
Title The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate)
Journal name Journal of Shellfish Research   Check publisher's open access policy
ISSN 0730-8000
1943-6319
Publication date 2006
Year available 2006
Sub-type Article (original research)
DOI 10.2983/0730-8000(2006)25[631:TEOHCA]2.0.CO;2
Volume 25
Issue 2
Start page 631
End page 637
Total pages 7
Place of publication Hanover, PA, United States
Publisher National Shellfisheries Association
Collection year 2006
Language eng
Subject C1
270805 Genetic Engineering and Enzyme Technology
630303 Aquaculture
Abstract In this study tetraploid Marsupenaeus japonicus (Bate) embryos were produced by preventing the first division in mitosis. The effectiveness of temperature and chemical shocks for producing tetraploid M. japonicus were assessed when applied at different times postspawning and for different durations. Tetraploid M. japonicus embryos (spawned at 27 degrees C) were produced by heat shocks at 35 degrees C and 36 degrees C in three and eight spawning samples respectively, and a cold shock at 5 degrees C in a single spawning sample. All temperature shocks inducing tetraploidy were applied 18-23 min postspawning for a 5-10 min duration. The percentage of spawnings successfully inducing tetraploid embryos (i.e., frequency of induction) ranged from 33.33% to 66.67% for the 21, 22 and 23 min postspawning heat shock treatment regimes. The percentage of tetraploid embryos within an induction (i.e., induction rate), as determined by flow cytometry, ranged from 8.82% to 98.12% (ave. S.E.) (34.4 +/- 21.4%) for the 35 degrees C shock treatments, from 13.12% to 61.02% (35.0 +/- 5.0%) for the 36 degrees C shock treatments and was 15% for the 5 degrees C cold shock treatment. No tetraploids were produced for spawnings that received heat shocks above 36 degrees C or below 35 degrees C, or for cold shocks above 5 degrees C for any of the tested postspawning treatment and duration times. Chemical shock with 150 mu M 6-dimethylaminopurine did not result in tetraploid M. japonicus embryos at any of the tested postspawning treatment times and durations. Tetraploid M. japonicus embryos were nonviable, with no tetraploid larvae being detected by flow cytometry. Based on our results heat shocking of M. japonicus embryos at 36 degrees C, 23 min postspawning for a 5-10 min duration is the most effective means to produce tetraploids through inhibition of the first mitotic division (taking into consideration the importance of frequency and induction rate equally).
Keyword Penaeus Japonicus
Polyploidy
Shrimp
Selective Breeding
Genetic Protection
Fisheries
Marine & Freshwater Biology
Crassostrea-gigas Thunberg
Pacific Oyster
Induction
Carp
Embryos
Eggs
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 09:20:22 EST