The detection of environmental autoinducing peptide quorum-sensing genes from an uncultured Clostridium sp in landfill leachate reactor biomass

Burrell, P. C. (2006) The detection of environmental autoinducing peptide quorum-sensing genes from an uncultured Clostridium sp in landfill leachate reactor biomass. Letters In Applied Microbiology, 43 4: 455-460. doi:10.1111/j.1472-765X.2006.01968.x


Author Burrell, P. C.
Title The detection of environmental autoinducing peptide quorum-sensing genes from an uncultured Clostridium sp in landfill leachate reactor biomass
Journal name Letters In Applied Microbiology   Check publisher's open access policy
ISSN 0266-8254
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1111/j.1472-765X.2006.01968.x
Volume 43
Issue 4
Start page 455
End page 460
Total pages 6
Place of publication Oxford
Publisher Blackwell Publishing
Collection year 2006
Language eng
Subject C1
270399 Microbiology not elsewhere classified
780105 Biological sciences
Abstract Aims: To elucidate whether a dominant uncultured clostridial (Clostridium thermocellum-like) species in an environmental sample (landfill leachate), possesses an autoinducing peptide (AIP) quorum-sensing (QS) gene, although it may not be functional. Methods and Results: A modified AIP accessory gene regulator (agr)C PCR protocol was performed on extracted DNA from a landfill leachate sample (also characterized by 16S rRNA gene cloning) and the PCR products were cloned, sequenced and phylogenetically analysed. It appeared that two agrC gene phylotypes existed, most closely related to the C. thermocellum agrC gene, differing by only 1 bp. Conclusions: It is possible to specifically identify and characterize the agrC AIP QS gene from uncultured Firmicutes (C. thermocellum-like) bacteria derived from environmental (landfill leachate) sample. Significance and Impact of the Study: This is the first successful attempt at identifying AIP QS genes from a cellulolytic environment (landfill). The agrC gene was identified as being most closely related to the C. thermocellum agrC gene, the same bacterium identified as being dominant, according to 16S rRNA gene cloning and subsequently fluorescence in situ hybridization analyses, in the same biomass.
Keyword anaerobic
autoinducing peptide
Clostridium thermocellum
landfill
microbial ecology
quorum sensing
Gram-positive Bacteria
To-cell Communication
Staphylococcus-aureus
Signal Molecules
Rumen Bacteria
Identification
Biofilms
Expression
Community
Virulence
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2007 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 15 Aug 2007, 19:19:38 EST