Strategies to obtain stable transgenic plants from non-embryogenic lines: complementation of the nn(1) mutation of the NORK gene in Medicago sativa MN1008

Perhald, Ariana, Endre, Gabriella, Kevei, Zoltan, Kiss, Gyorgy B. and Kereszt, Attila (2006) Strategies to obtain stable transgenic plants from non-embryogenic lines: complementation of the nn(1) mutation of the NORK gene in Medicago sativa MN1008. Plant Cell Reports, 25 8: 799-806. doi:10.1007/s00299-006-0117-6


Author Perhald, Ariana
Endre, Gabriella
Kevei, Zoltan
Kiss, Gyorgy B.
Kereszt, Attila
Title Strategies to obtain stable transgenic plants from non-embryogenic lines: complementation of the nn(1) mutation of the NORK gene in Medicago sativa MN1008
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1007/s00299-006-0117-6
Volume 25
Issue 8
Start page 799
End page 806
Total pages 8
Place of publication New York
Publisher Springer
Collection year 2006
Language eng
Subject C1
270803 Transgenesis
620108 Grain legumes
Abstract Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the recessive non-nodulating mutant allele nn(1) was crossed with the highly embryogenic alfalfa line Regen S and embryogenic hybrid individuals were identified from the F1 progeny. After transformation of these hybrids with the wild-type gene (NORK), an F2 generation segregating for the mutation and transgene were produced. Plants homozygous for the mutant allele and carrying the wild-type NORK transgene could form root nodules after inoculation with Sinorhizobium meliloti demonstrating successful complementation of the nn(1) mutation.
Keyword Medicago
Stable Transformation
Embryogenicity
Nodulation
Mutant
Complementation
Plant Sciences
Somatic Embryogenesis
Non-nodulation
Ineffective Nodulation
Rhizobium-meliloti
Nodule Development
Root Hairs
Alfalfa
Construction
Registration
Inheritance
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
2007 Higher Education Research Data Collection
 
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Created: Wed, 15 Aug 2007, 19:16:08 EST