Sequencing a new target genome: The Boophilus microplus (Acari : Ixodidae) genome project

Guerrero, Felix D., Nene, Vishvanath M., George, John E., Barker, Stephen C. and Willadsen, Peter (2006) Sequencing a new target genome: The Boophilus microplus (Acari : Ixodidae) genome project. Journal of Medical Entomology, 43 1: 9-16. doi:10.1603/0022-2585(2006)043[0009:SANTGT]2.0.CO;2

Author Guerrero, Felix D.
Nene, Vishvanath M.
George, John E.
Barker, Stephen C.
Willadsen, Peter
Title Sequencing a new target genome: The Boophilus microplus (Acari : Ixodidae) genome project
Formatted title
Sequencing a new target genome: The Boophilus microplus (Acari : Ixodidae) genome project
Journal name Journal of Medical Entomology   Check publisher's open access policy
ISSN 0022-2585
Publication date 2006-01
Sub-type Article (original research)
DOI 10.1603/0022-2585(2006)043[0009:SANTGT]2.0.CO;2
Volume 43
Issue 1
Start page 9
End page 16
Total pages 8
Place of publication Lanham, MD, U.S.A.
Publisher Entomological Society America
Collection year 2007
Language eng
Subject CX
270202 Genome Structure
780105 Biological sciences
Abstract The southern cattle tick, Boophilus microplus (Canestrini), causes annual economic losses in the hundreds of millions of dollars to cattle producers throughout the world, and ranks as the most economically important tick from a global perspective. Control failures attributable to the development of pesticide resistance have become commonplace, and novel control technologies are needed. The availability of the genome sequence will facilitate the development of these new technologies, and we are proposing sequencing to a 4-6X draft coverage. Many existing biological resources are available to facilitate a genome sequencing project, including several inbred laboratory tick strains, a database of approximate to 45,000 expressed sequence tags compiled into a B. microplus Gene Index, a bacterial artificial chromosome (BAC) library, an established B. microplus cell line, and genomic DNA suitable for library synthesis. Collaborative projects are underway to map BACs and cDNAs to specific chromosomes and to sequence selected BAC clones. When completed, the genome sequences from the cow, B. microphis, and the B. microphis-borne pathogens Babesia bovis and Anaplasma marginale will enhance studies of host-vector-pathogen systems. Genes involved in the regeneration of amputated tick limbs and transitions through developmental stages are largely unknown. Studies of these and other interesting biological questions will be advanced by tick genome sequence data. Comparative genomics offers the prospect of new insight into many, perhaps all, aspects of the biology of ticks and the pathogens they transmit to farm animals and people. The B. microplus genome sequence will fill a major gap in comparative genomics: a sequence from the Metastriata lineage of ticks. The purpose of the article is to synergize interest in and provide rationales for sequencing the genome of B. microplus and for publicizing currently available genomic resources for this tick.
Keyword Boophilus Microplus
Genome Sequence
Gene Identification
Babesia Bovis
Molecular Genetics
Veterinary Sciences
Putative Acetylcholinesterase Cdna
Tick Amblyomma-americanum
Salivary-gland Extracts
Southern Cattle Tick
Rna Interference
Pyrethroid Resistance
Mexican Strains
Q-Index Code CX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under 'Forum'.

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Created: Wed, 15 Aug 2007, 09:13:21 EST