Scube2 mediates Hedgehog signalling in the zebrafish embryo

Hollway, G. E., Maule, J., Gautier, P., Evans, T. M., Keenan, D. G., Lohs, C., Fischer, D., Wicking, C. and Currie, P. D. (2006) Scube2 mediates Hedgehog signalling in the zebrafish embryo. Developmental Biology, 294 1: 104-118. doi:10.1016/j.ydbio.2006.02.032


Author Hollway, G. E.
Maule, J.
Gautier, P.
Evans, T. M.
Keenan, D. G.
Lohs, C.
Fischer, D.
Wicking, C.
Currie, P. D.
Title Scube2 mediates Hedgehog signalling in the zebrafish embryo
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2006.02.032
Volume 294
Issue 1
Start page 104
End page 118
Total pages 15
Place of publication San Diego
Publisher Academic Press
Collection year 2006
Language eng
Subject C1
270106 Cell Development (incl. Cell Division and Apoptosis)
780105 Biological sciences
1103 Clinical Sciences
Abstract The Hedgehog family of secreted morphogens specifies the fate of a large number of different cell types within invertebrate and vertebrate embryos, including the muscle cell precursors of the embryonic myotome of zebrafish. Formation of Hedgehog-sensitive muscle fates is disrupted within homozygous zebrafish mutants of the you-type class, the majority of which disrupt components of the Hedgehog (HH) signal transduction pathway. We have undertaken a phenotypic and molecular characterisation of one of these mutants, you, which we show results from mutations within the zebrafish orthologue of the mammalian, gene scube2. This gene encodes a member of the Scube family of proteins, which is characterised by several protein motifs including EGF and CUB domains. Epistatic and molecular analyses position Scube2 function upstream of Smoothened (Smoh), the signalling component of the HH receptor complex, suggesting that Scube2 may act during HH signal transduction prior to, or during, receipt of the HH signal at the plasma membrane. In support of this model we show that scube2 has homology to cubilin, which encodes an endocytic receptor involved in protein trafficking suggesting a possible mode of function for Scube2 during HH signal transduction. (c) 2006 Elsevier Inc. All rights reserved.
Keyword Scube2
Hedgehog
Zebrafish
Developmental Biology
Muscle-cell-type
Sonic-hedgehog
Drosophila Ortholog
Negative Regulator
Floor Plate
Dally-like
Induction
Protein
Mutations
Megalin
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 19:12:38 EST