Recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum

Gan, Xiao-Xian, Shen, Li-Ying, Wang, Yue, Ding, Jian-Zu, Shen, Hui-Ying, Zeng, Xiao-Peng, McManus, Donald P., Brindley, Paul J. and Fan, Jinjiang (2006) Recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum. Memorias Do Instituto Oswaldo Cruz, 101 1: 9-13. doi:10.1590/S0074-02762006000100003


Author Gan, Xiao-Xian
Shen, Li-Ying
Wang, Yue
Ding, Jian-Zu
Shen, Hui-Ying
Zeng, Xiao-Peng
McManus, Donald P.
Brindley, Paul J.
Fan, Jinjiang
Title Recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum
Formatted title
Recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum
Journal name Memorias Do Instituto Oswaldo Cruz   Check publisher's open access policy
ISSN 0074-0276
1678-8060
Publication date 2006-02
Sub-type Article (original research)
DOI 10.1590/S0074-02762006000100003
Open Access Status DOI
Volume 101
Issue 1
Start page 9
End page 13
Total pages 5
Place of publication Rio De Janeiro, RJ, Brazil
Publisher Fundaco Oswaldo Cruz
Collection year 2006
Language eng
Subject C1
Formatted abstract
A polyhistidine-tagged recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein (SVLBP) from adult Schistosoma japonicum was expressed in Escherichia coli. The affinity purified rSVLBP was used to vaccinate mice. The worm numbers and egg deposition recovered from the livers and veins of the immunized mice were 33.5% and 47.6% less than that from con trol mice, respectively (p < 0.05). There was also a marked increase in the antibody response in vaccinated mice: the titer of IgG1 and IgG2a, IgG2b in the vaccinated group was significantly big-her than that in the controls (> 1:6,400 in total IgG). In a comparison of the reactivity of sera from healthy individuals and patients with rSVLBP, recognition patterns against this parasite tegumental antigen varied among different groups of the individuals Notably, the average titres of anti-rSVLBP antibody in sera from faecal egg-negative individuals was significantly higher than that in sera from the faecal egg-positives, which maybe reflect SVLBP-specjfic protection. These results suggested that the parasite tegumentalprotein SVLBP was a promising candidate for further investigation as a vaccine antigen for use against Asian schistosomiasis.
Keyword Schistosoma japonicum
Schistosoma japonicum very lowdensity lipoprotein binding protein
vaccine
Host-parasite Interactions
Protective Immunity
Defined Population
Native Antigens
In-vitro
Mansoni
Immunization
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2007 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 09:09:32 EST