Recognition and inactivation of LPS by lipophorin particles

Ma, Gang, Hay, Douglas, Li, Dongmei, Asgari, Sassan and Schmidt, Otto (2006) Recognition and inactivation of LPS by lipophorin particles. Developmental And Comparative Immunology, 30 7: 619-626. doi:10.1016/j.dci.2005.09.003


Author Ma, Gang
Hay, Douglas
Li, Dongmei
Asgari, Sassan
Schmidt, Otto
Title Recognition and inactivation of LPS by lipophorin particles
Journal name Developmental And Comparative Immunology   Check publisher's open access policy
ISSN 0145-305X
Publication date 2006
Sub-type Article (original research)
DOI 10.1016/j.dci.2005.09.003
Volume 30
Issue 7
Start page 619
End page 626
Total pages 8
Place of publication Oxford, England
Publisher Pergamon-Elsevier Science Ltd
Collection year 2006
Language eng
Subject C1
270504 Invertebrate Biology
780105 Biological sciences
Abstract Lipophorin is the major lipid carrier in insects, but various observations indicate that lipophorin is also involved in immune reactions. To examine a possible role of lipophorin in defence reactions, we mixed hemolymph plasma from Galleria mellonella with LPS and noticed that lipophorin forms detergent-insoluble aggregates, while most other plasma proteins are not affected. Lipophorin particles isolated by low-density gradient centrifugation retained LPS-induced aggregation properties, which suggested to us that these immune-reactive particles are able to recognise LPS and respond by forming insoluble aggregates. Antibodies against LPS-binding proteins, such as immulectin-2 and beta-1,3-glucan binding protein, cross-reacted with proteins associated with purified lipophorin particles. To examine whether LPS-mediated aggregates inactivate LPS, we added LPS-lipophorin mixtures to purified lipophorin particles and monitored aggregate formation. Under these conditions lipophorin did not form insoluble aggregates, which indicates that lipophorin particles sequester LPS into non-toxic aggregates. (c) 2005 Elsevier Ltd. All rights reserved.
Keyword Immunology
Zoology
lipopolysaccharide
lipophorin
coagulation
LPS binding protein
Galleria mellonella
Immunoglobulin Superfamily
Insect Hemolymph
Lipid Transport
Manduca-sexta
Protein
Coagulation
Hemolin
Lipoprotein
Endotoxin
Detoxification
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 09:09:30 EST