The asymptomatic bacteriuria Escherichia coli strain 83972 outcompetes uropathogenic E-coli strains in human urine

Roos, Viktoria, Ulett, Glen C., Schembri, Mark A. and Klemm, Per (2006) The asymptomatic bacteriuria Escherichia coli strain 83972 outcompetes uropathogenic E-coli strains in human urine. Infection and Immunity, 74 1: 615-624. doi:10.1128/IAI.74.1.615-624.2006

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Author Roos, Viktoria
Ulett, Glen C.
Schembri, Mark A.
Klemm, Per
Title The asymptomatic bacteriuria Escherichia coli strain 83972 outcompetes uropathogenic E-coli strains in human urine
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2006-01
Sub-type Article (original research)
DOI 10.1128/IAI.74.1.615-624.2006
Open Access Status File (Publisher version)
Volume 74
Issue 1
Start page 615
End page 624
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2006
Language eng
Abstract Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU). In contrast to uropathogenic E. coli (UPEC), which causes symptomatic urinary tract infections (UTI), very little is known about the mechanisms by which these strains colonize the human urinary tract. The prototype ABU E. coli strain 83972 was originally isolated from a girl who had carried it asymptomatically for 3 years. Deliberate colonization of UTI-susceptible individuals with E. coli 83972 has been used successfully as an alternative approach for the treatment of patients who are refractory to conventional therapy. Colonization with strain 83972 appears to prevent infection with UPEC strains in such patients despite the fact that this strain is unable to express the primary adhesins involved in UTI, viz. P and type 1 fimbriae. Here we investigated the growth characteristics of E. coli 83972 in human urine and show that it can outcompete a representative spectrum of UPEC strains for growth in urine. The unique ability of ABU E. coli 83972 to outcompete UPEC in urine was also demonstrated in a murine model of human UTI, confirming the selective advantage over UPEC in vivo. Comparison of global gene expression profiles of E. coli 83972 grown in lab medium and human urine revealed significant differences in expression levels in the two media; significant down-regulation of genes encoding virulence factors such as hemolysin, lipid A, and capsular pollysaccharides was observed in cells grown in urine. Clearly, divergent abilities of ABU E. coli and UPEC to exploit human urine as a niche for persistence and survival suggest that these key differences may be exploited for preventative and/or therapeutic approaches.
Keyword Immunology
Infectious Diseases
Tract-infection
Virulence
Expression
Hemolysin
Fimbriae
Epidemiology
Microarray
Adherence
Transport
Adhesin
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 09:06:19 EST