PerR controls Mn-dependent resistance to oxidative stress in Neisseria gonorrhoeae

Wu, H. J., Seib, K. L., Srikhanta, Y. N., Kidd, S. P., Edwards, J. L., Maguire, T. L., Grimmond, S. M., Apicella, M. A., McEwan, A. G. and Jennings, M. P. (2006) PerR controls Mn-dependent resistance to oxidative stress in Neisseria gonorrhoeae. Molecular Microbiology, 60 2: 401-416. doi:10.1111/j.1365-2958.2006.05079.x


Author Wu, H. J.
Seib, K. L.
Srikhanta, Y. N.
Kidd, S. P.
Edwards, J. L.
Maguire, T. L.
Grimmond, S. M.
Apicella, M. A.
McEwan, A. G.
Jennings, M. P.
Title PerR controls Mn-dependent resistance to oxidative stress in Neisseria gonorrhoeae
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
Publication date 2006
Sub-type Article (original research)
DOI 10.1111/j.1365-2958.2006.05079.x
Volume 60
Issue 2
Start page 401
End page 416
Total pages 16
Place of publication Oxford
Publisher Blackwell Publishing
Collection year 2006
Language eng
Subject C1
270301 Bacteriology
730101 Infectious diseases
Abstract In previous studies it has been established that resistance to superoxide by Neisseria gonorrhoeae is dependent on the accumulation of Mn(II) ions involving the ABC transporter, MntABC. A mutant strain lacking the periplasmic binding protein component (MntC) of this transport system is hypersensitive to killing by superoxide anion. In this study the mntC mutant was found to be more sensitive to H2O2 killing than the wild-type. Analysis of regulation of MntC expression revealed that it was de-repressed under low Mn(II) conditions. The N. gonorrhoeae mntABC locus lacks the mntR repressor typically found associated with this locus in other organisms. A search for a candidate regulator of mntABC expression revealed a homologue of PerR, a Mn-dependent peroxide-responsive regulator found in Gram-positive organisms. A perR mutant expressed more MntC protein than wild-type, and expression was independent of Mn(II), consistent with a role for PerR as a repressor of mntABC expression. The PerR regulon of N. gonorrhoeae was defined by microarray analysis and includes ribosomal proteins, TonB-dependent receptors and an alcohol dehydrogenase. Both the mntC and perR mutants had reduced intracellular survival in a human cervical epithelial cell model.
Keyword Biochemistry & Molecular Biology
Microbiology
Global Transcriptional Response
Escherichia-coli
Bacillus-subtilis
Hydrogen-peroxide
Superoxide-dismutase
Salmonella-typhimurium
Epithelial-cells
Heat-shock
Streptomyces-coelicolor
Streptococcus-pyogenes
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 09:03:21 EST