Nucleic acid amplification testing for Neisseria gonorrhoeae: An ongoing challenge

Whiley, David M., Tapsall, John W. and Sloots, Theo P. (2006) Nucleic acid amplification testing for Neisseria gonorrhoeae: An ongoing challenge. Journal of Molecular Diagnostics, 8 1: 3-15. doi:10.2353/jmoldx.2006.050045

Author Whiley, David M.
Tapsall, John W.
Sloots, Theo P.
Title Nucleic acid amplification testing for Neisseria gonorrhoeae: An ongoing challenge
Journal name Journal of Molecular Diagnostics   Check publisher's open access policy
ISSN 1525-1578
Publication date 2006
Sub-type Article (original research)
DOI 10.2353/jmoldx.2006.050045
Volume 8
Issue 1
Start page 3
End page 15
Total pages 13
Editor Karen L. Kaul
Place of publication Bethesda, Maryland, U.S.A.
Publisher American Society Investigative Pathology & The Association for Molecular Pathology
Collection year 2006
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract Nucleic acid amplification tests (NAATs) for the detection of Neisseria gonorrhoeae became available in the early 1990s. Although offering several advantages over traditional detection methods, N. gonorrhoeae NAATs do have some limitations. These include cost, risk of carryover contamination, inhibition, and inability to provide antibiotic resistance data. In addition, there are sequence-related limitations that are unique to N. gonorrhoeae NAATs. In particular, false-positive results are a major consideration. These primarily stem from the frequent horizontal genetic exchange occurring within the Neisseria genus, leading to commensal Neisseria species acquiring N. gonorrhoeae genes. Furthermore, some N. gonorrhoeae subtypes may lack specific sequences targeted by a particular NAAT. Therefore, NAAT false-negative results because of sequence variation may occur in some gonococcal populations. Overall, the N. gonorrhoeae species continues to present a considerable challenge for molecular diagnostics. The need to evaluate N. gonorrhoeae NAATs before their use in any new patient population and to educate physicians on the limitations of these tests is emphasized in this review.
Keyword Nucleic Acid Amplification Testing
Neisseria Gonorrhoeae
Chlamydia-trachomatis Infection
Real-time Pcr
Strand Displacement Amplification
Sexually-transmitted Diseases
Endocervical Swab Specimens
Positive Predictive-value
Low-prevalence Population
Cost-saving Strategy
Amplicor Ct/ng Tests
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 09:00:16 EST