Immunogenicity of liposomes containing lipid core peptides and the adjuvant Quil A

White, Karen, Rades, Thomas, Kearns, Philip, Toth, Istvan and Hook, Sarah (2006) Immunogenicity of liposomes containing lipid core peptides and the adjuvant Quil A. Pharmaceutical Research, 23 7: 1473-1481. doi:10.1007/s11095-006-0272-z

Author White, Karen
Rades, Thomas
Kearns, Philip
Toth, Istvan
Hook, Sarah
Title Immunogenicity of liposomes containing lipid core peptides and the adjuvant Quil A
Journal name Pharmaceutical Research   Check publisher's open access policy
ISSN 0724-8741
Publication date 2006
Sub-type Article (original research)
DOI 10.1007/s11095-006-0272-z
Volume 23
Issue 7
Start page 1473
End page 1481
Total pages 9
Place of publication New York
Publisher Springer/Plenum Publishers
Collection year 2006
Language eng
Subject C1
250302 Biological and Medical Chemistry
730108 Cancer and related disorders
Abstract Purpose. The purpose of this study was to investigate the immunogenicity of liposomes containing mannosylated lipid core peptide (manLCP) constructs, both in vitro and in vivo, with or without the addition of the immune stimulating adjuvant Quil A. Methods. Mouse bone marrow dendritic cells (BMDC) were cultured with liposome formulations for 48 h, and the resulting level of BMDC activation was determined by flow cytometry. BMDC pulsed with liposome formulations were incubated with 5,6-carboxyfluoroscein diacetate succinimidyl ester-labeled T cells for 72 h and the resulting T cell proliferation was determined by flow cytometry. To investigate the immunogenicity of formulations in vivo, groups of C57Bl/6J mice were immunized by subcutaneous injection, and the resulting antigen-specific cytotoxic and protective immune responses toward tumor challenge evaluated. Results. Despite being unable to demonstrate the activation of BMDC, BMDC pulsed with liposomes containing manLCP constructs were able to stimulate the proliferation of naive T cells in vitro. However, in vivo only liposomes containing both manLCP and Quil A were able to stimulate a strong antigen-specific cytotoxic immune response. Liposomes containing manLCP and Quil A within the same particle were able to protect against the growth of tumor cells to a similar level as if the antigen was administered in alum with CD4 help. Conclusion. ManLCPs administered in liposomes are able to stimulate strong cytotoxic and protective immune responses if Quil A is also incorporated as an adjuvant.
Keyword Cd8 T Cell
Lipid Core Peptide
Quil A
Chemistry, Multidisciplinary
Pharmacology & Pharmacy
Dendritic Cell-receptor
Cd8(+) T-cells
Antigen Presentation
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 08:59:01 EST