Microbial diversity within the water column of a larval rearing system for the ornate rock lobster (Panulirus ornatus)

Payne, Matthew S., Hall, Mike R., Bannister, Raymond, Sly, Lindsay and Bourne, David G. (2006) Microbial diversity within the water column of a larval rearing system for the ornate rock lobster (Panulirus ornatus). Aquaculture, 258 1-4: 80-90. doi:10.1016/j.aquaculture.2006.04.001


Author Payne, Matthew S.
Hall, Mike R.
Bannister, Raymond
Sly, Lindsay
Bourne, David G.
Title Microbial diversity within the water column of a larval rearing system for the ornate rock lobster (Panulirus ornatus)
Journal name Aquaculture   Check publisher's open access policy
ISSN 0044-8486
1873-5622
Publication date 2006
Sub-type Article (original research)
DOI 10.1016/j.aquaculture.2006.04.001
Volume 258
Issue 1-4
Start page 80
End page 90
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2006
Language eng
Subject C1
270307 Microbial Ecology
630303 Aquaculture
Abstract The ornate tropical rock lobster, Panulirus ornatus has substantial potential as an aquaculture species though disease outbreaks during the animal's extended larval lifecycle are major constraints for success. In order to effectively address such disease-related issues, an improved understanding of the composition and dynamics of the microbial communities in the larval rearing tanks is required. This study used flow cytometry and molecular microbial techniques (clone libraries and denaturing gradient gel electrophoresis (DGGE)) to quantify and characterise the microbial community of the water column in the early stages (developmental stage I-II) of a P. ornatus larval rearing system. DGGE analysis of a 5000 L larval rearing trial demonstrated a dynamic microbial community with distinct changes in the community structure after initial stocking (day I to day 2) and from day 4 to day 5, after which the structure was relatively stable. Flow cytometry analysis of water samples taken over the duration of the trial demonstrated a major increase in bacterial load leading up to and peaking on the first day of the initial larval moult (day 7), before markedly decreasing prior to when > 50% of larvae moulted (day 9). A clone library of a day 10 water sample taken following a mass larval mortality event reflected high microbial diversity confirmed by statistical analysis indices. Sequences retrieved from both clone library and DGGE analyses were dominated by gamma- and alpha-Proteobacteria affiliated organisms with additional sequences affiliated with beta- and epsilon-Proteobacteria, Bacteroidetes, Cytophagales and Chlamydiales groups. Vibrio affiliated species were commonly retrieved in the clone library, though absent from DGGE analysis.
Keyword Panulirus Ornatus Phyllosoma
Bacterial Diversity
16s Rrna
Dgge
Clone Library
Flow Cytometry
Fisheries
Marine & Freshwater Biology
16s Ribosomal-rna
Gradient Gel-electrophoresis
Penaeus-monodon Fabricius
Polymerase-chain-reaction
Community Structure
Tiger Prawn
Bacterial Community
Genetic Diversity
Vibrio-harveyi
Population
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 08:55:45 EST