Mechanics of cellular adhesion to artificial artery templates

Knoner, Gregor, Rolfe, Barbara E., Campbell, Julie H., Parkin, Simon J., Heckenberg, Norman R. and Rubinsztein-Dunlop, Halina (2006) Mechanics of cellular adhesion to artificial artery templates. Biophysical Journal, 91 8: 3085-3096. doi:10.1529/biophysj.105.076125

Author Knoner, Gregor
Rolfe, Barbara E.
Campbell, Julie H.
Parkin, Simon J.
Heckenberg, Norman R.
Rubinsztein-Dunlop, Halina
Title Mechanics of cellular adhesion to artificial artery templates
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
Publication date 2006-10-15
Sub-type Article (original research)
DOI 10.1529/biophysj.105.076125
Volume 91
Issue 8
Start page 3085
End page 3096
Total pages 12
Editor Robert Callender
Place of publication United States
Publisher Biophysical Society
Collection year 2006
Language eng
Subject C1
249901 Biophysics
780102 Physical sciences
Abstract We are using polymer templates to grow artificial artery grafts in vivo for the replacement of diseased blood vessels. We have previously shown that adhesion of macrophages to the template starts the graft formation. We present a study of the mechanics of macrophage adhesion to these templates on a single cell and single bond level with optical tweezers. For whole cells, in vitro cell adhesion densities decreased significantly from polymer templates polyethylene to silicone to Tygon (167, 135, and 65 cells/mm(2)). These cell densities were correlated with the graft formation success rate (50%, 25%, and 0%). Single-bond rupture forces at a loading rate of 450 pN/s were quantified by adhesion of trapped 2-mm spheres to macrophages. Rupture force distributions were dominated by nonspecific adhesion (forces, < 40 pN). On polystyrene, preadsorption of fibronectin or presence of serum proteins in the cell medium significantly enhanced adhesion strength from a mean rupture force of 20 pN to 28 pN or 33 pN, respectively. The enhancement of adhesion by fibronectin and serum is additive (mean rupture force of 43 pN). The fraction of specific binding forces in the presence of serum was similar for polystyrene and polymethyl-methacrylate, but specific binding forces were not observed for silica. Again, we found correlation to in vivo experiments, where the density of adherent cells is higher on polystyrene than on silica templates, and can be further enhanced by fibronectin adsorption. These findings show that in vitro adhesion testing can be used for template optimization and to substitute for in-vivo experiments.
Keyword Biophysics
Atomic-force Microscopy
Optical Tweezers
Binding Strength
Living Cells
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 08:55:10 EST