Host B cells produce IL-10 following TBI and attenuate acute GVHD after allogeneic bone marrow transplantation

Rowe, V, Banovic, T, MacDonald, KP, Kuns, R, Don, AL, Morris, ES, Burman, AC, Bofinger, HM, Clouston, AD and Hill, GR (2006) Host B cells produce IL-10 following TBI and attenuate acute GVHD after allogeneic bone marrow transplantation. Blood, 108 7: 2485-2492. doi:10.1182/blood-2006-04-016063


Author Rowe, V
Banovic, T
MacDonald, KP
Kuns, R
Don, AL
Morris, ES
Burman, AC
Bofinger, HM
Clouston, AD
Hill, GR
Title Host B cells produce IL-10 following TBI and attenuate acute GVHD after allogeneic bone marrow transplantation
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
Publication date 2006
Sub-type Article (original research)
DOI 10.1182/blood-2006-04-016063
Volume 108
Issue 7
Start page 2485
End page 2492
Total pages 8
Editor S. J. Shattil
Place of publication USA
Publisher American Society of Hematology
Collection year 2006
Language eng
Subject C1
320205 Transplantation Immunology
730102 Immune system and allergy
1103 Clinical Sciences
Abstract Host antigen-presenting cells (APCs) are known to be critical for the induction of graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation (BMT), but the relative contribution of specific APC subsets remains unclear. We have studied the role of host B cells in GVHD by using B-cell-deficient mu MT mice as BMT recipients in a model of CD4-dependent GVHD to major histocompatlibility complex antigens. We demonstrate that acute GVHD is initially augmented in mu MT recipients relative to wild-type recipients (mortality: 85% vs 44%, P < .01), and this is the result of an increase in donor T-cell proliferation, expansion, and inflammatory cytokine production early after BMT. Recipient B cells were depleted 28-fold at the time of BMT by total body irradiation (TBI) administered 24 hours earlier, and we demonstrate that TBI rapidly induces sustained interleukin-110 (IL-10) generation from B cells but not dendritic cells (DCs) or other cellular populations within the spleen. Finally, recipient mice in which B cells are unable to produce IL-10 due to homologous gene deletion develop more severe acute GVHD than recipient mice in which B cells are wild type. Thus, the induction of IL-10 in host B cells during conditioning attenuates experimental acute GVHD.
Keyword Hematology
Regulatory T-cells
Antigen-presenting Cells
Dendritic Cells
Disease
Mice
Lymphocytes
Cd4(+)
Irradiation
Gamma
Gene
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2007 Higher Education Research Data Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 62 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 66 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 08:48:51 EST