Glucosamine supplementation during in vitro maturation inhibits subsequent embryo development: Possible role of the of developmental competence

Sutton-McDowall, M. L., Mitchell, M., Cetica, P., Dalvit, G., Pantaleon, M., Lane, M., Gilchrist, R. B. and Thompson, J. G. (2006) Glucosamine supplementation during in vitro maturation inhibits subsequent embryo development: Possible role of the of developmental competence. Biology Of Reproduction, 74 5: 881-888. doi:10.1095/biolreprod.105.048553


Author Sutton-McDowall, M. L.
Mitchell, M.
Cetica, P.
Dalvit, G.
Pantaleon, M.
Lane, M.
Gilchrist, R. B.
Thompson, J. G.
Title Glucosamine supplementation during in vitro maturation inhibits subsequent embryo development: Possible role of the of developmental competence
Journal name Biology Of Reproduction   Check publisher's open access policy
ISSN 0006-3363
Publication date 2006
Sub-type Article (original research)
DOI 10.1095/biolreprod.105.048553
Volume 74
Issue 5
Start page 881
End page 888
Total pages 8
Editor John Eppig
Mary Ann Handel
Place of publication Madison
Publisher Soc Study Reproduction
Collection year 2006
Language eng
Subject C1
730116 Reproductive system and disorders
Abstract Glucose concentration during cumulus-oocyte complex (COC) maturation influences several functions, including progression of oocyte meiosis, oocyte developmental competence, and cumulus mucification. Glucosamine (GlcN) is an alternative hexose substrate, specifically metabolized through the hexosamine biosynthesis pathway, which provides the intermediates for extracellular matrix formation during cumulus cell mucification. The aim of this study was to determine the influence of GlcN on meiotic progression and oocyte developmental competence following in vitro maturation (IVM). The presence of GlcN during bovine IVM did not affect the completion of nuclear maturation and early cleavage, but severely perturbed blastocyst development. This effect was subsequently shown to be dose-dependent and was also observed for porcine oocytes matured in vitro. Hexosamine biosynthesis upregulation using GlcN supplementation is well known to increase O-linked glycosylation of many intracellular signaling molecules, the best-characterized being the phosphoinositol-3-kinase (PI3K) signaling pathway. We observed extensive O-linked glycosylation in bovine cumulus cells, but not oocytes, following IVM in either the presence or the absence of GlcN. Inhibition of O-linked glycosylation significantly reversed the effect of GlcN-induced reduction in developmental competence, but inhibition of PI3K signaling had no effect. Our data are the first to link hexosamine biosynthesis, involved in cumulus cell mucification, to oocyte developmental competence during in vitro maturation.
Keyword cumulus
cumulus cells
embryo
early development
glucose
glucosamine
hexosamine
oocyte
oocyte development
Induce Insulin-resistance
Hexosamine Pathway Leads
Cumulus Cell Complexes
O-glcnac Transferase
Tetratricopeptide Repeats
Different Mechanisms
3t3-l1 Adipocytes
Oocyte Maturation
Hyaluronic-acid
Bovine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 08:48:25 EST