Genetic manipulation of blood group carbohydrates alters development and pathfinding of primary sensory axons of the olfactory systems

St John, James A., Claxton, Christina, Robinson, Mark W., Yamamoto, Fumiichiro, Domino, Steven E. and Key, Brian (2006) Genetic manipulation of blood group carbohydrates alters development and pathfinding of primary sensory axons of the olfactory systems. Developmental Biology, 298 2: 470-484. doi:10.1016/j.ydbio.2006.06.052


Author St John, James A.
Claxton, Christina
Robinson, Mark W.
Yamamoto, Fumiichiro
Domino, Steven E.
Key, Brian
Title Genetic manipulation of blood group carbohydrates alters development and pathfinding of primary sensory axons of the olfactory systems
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
Publication date 2006-10-15
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2006.06.052
Volume 298
Issue 2
Start page 470
End page 484
Total pages 15
Editor Eric N. Olsen
Place of publication San Diego, CA, U.S.A.
Publisher Academic Press Inc Elsevier Science
Collection year 2006
Language eng
Subject C1
320602 Cell Physiology
730110 Respiratory system and diseases (incl. asthma)
Abstract Primary sensory neurons in the vertebrate olfactory systems are characterised by the differential expression of distinct cell surface carbohydrates. We show here that the histo-blood group H carbohydrate is expressed by primary sensory neurons in both the main and accessory olfactory systems while the blood group A carbohydrate is expressed by a subset of vomeronasal neurons in the developing accessory olfactory system. We have used both loss-of-function and gain-of-function approaches to manipulate expression of these carbohydrates in the olfactory system. In null mutant mice lacking the alpha(1,2)fucosyltransferase FUT1, the absence of blood group H carbohydrate resulted in the delayed maturation of the glomerular layer of the main olfactory bulb. In addition, ubiquitous expression of blood group A on olfactory axons in gain-of-function transgenic mice caused mis-routing of axons in the glomerular layer of the main olfactory bulb and led to exuberant growth of vomeronasal axons in the accessory olfactory bulb. These results provide in vivo evidence for a role of specific cell surface carbohydrates during development of the olfactory nerve pathways. (c) 2006 Elsevier Inc. All rights reserved.
Keyword Carbohydrate
Blood Group A
Blood Group H
Neuron
Guidance
Glomerulus
Accessory Olfactory
Developmental Biology
Cell-adhesion Molecule
Marker Protein
Monoclonal-antibody
Vomeronasal System
Epithelial-cells
Receptor Neurons
Skeletal-muscle
Polysialic Acid
Mice Deficient
N-cam
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 08:47:26 EST