Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections

Arden, Katherine E., McErlean, Peter, Nissen, Michael D, Sloots, Theo P. and Mackay, Ian M. (2006) Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections. Journal of Medical Virology, 78 9: 1232-1240. doi:10.1002/jmv.20689


Author Arden, Katherine E.
McErlean, Peter
Nissen, Michael D
Sloots, Theo P.
Mackay, Ian M.
Title Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections
Journal name Journal of Medical Virology   Check publisher's open access policy
ISSN 0146-6615
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1002/jmv.20689
Volume 78
Issue 9
Start page 1232
End page 1240
Total pages 9
Editor Arie J Zuckerman
Editor in Chief
Place of publication Hoboken USA
Publisher Wiley-Liss
Collection year 2006
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract Viruses are the major cause of pediatric acute respiratory tract infection (ARTI) and yet many suspected cases of infection remain uncharacterized. We employed 17 PCR assays and retrospectively screened 315 specimens selected by season from a predominantly pediatric hospital-based population. Before the Brisbane respiratory virus research study commenced, one or more predominantly viral pathogens had been detected in 15.2% (n = 48) of all specimens. The Brisbane study made an additional 206 viral detections, resulting in the identification of a microbe in 67.0% of specimens. After our study, the majority of microbes detected were RNA viruses (89.9%). Overall, human rhinoviruses (HRVs) were the most frequently identified target (n=140) followed by human adenoviruses (HAdVs; n = 25), human metapneumovirus (HMPV; n=18), human bocavirus (HBoV; n = 15), human respiratory syncytial virus (HRSV; n = 12), human coronaviruses (HCoVs; n = 11), and human herpesvirus-6 (n = 11). HRVs were the sole microbe detected in 37.8% (n = 31) of patients with suspected lower respiratory tract infection (LRTI). Genotyping of the HRV VP4/VP2 region resulted in a proposed subdivision of HRV type A into sublineages A1 and A2. Most of the genotyped HAdV strains were found to be type C. This study describes the high microbial burden imposed by HRVs, HMPV, HRSV, HCoVs, and the newly identified virus, HBoV on a predominantly paediatric hospital population with suspected acute respiratory tract infections and proposes a new formulation of viral targets for future diagnostic research studies.
Keyword Virus
Respiratory Infection
Epidemiology
Rt-pcr
Diagnostics
Virology
Polymerase-chain-reaction
Transcriptase-pcr Assay
Real-time Pcr
Clinical Specimens
Sequence-analysis
Syncytial-virus
Diagnosis
Children
Samples
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 18:46:35 EST