Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target

Whiley, David M., Anderson, Trevor P., Barratt, Kevin, Beaman, Miles H., Buda, Philip J., Carter, Martine, Freeman, Kevin, Hallsworth, Peter, Limnios, E. Athena, Lum, Gary, Merien, Fabrice, Vernel-Pauillac, Frédérique, Tapsall, John W., Witt, Michael J., Nissen, Michael D. and Sloots, Theo P. (2006) Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target. Pathology, 38 5: 445-448. doi:10.1080/00313020600928253


Author Whiley, David M.
Anderson, Trevor P.
Barratt, Kevin
Beaman, Miles H.
Buda, Philip J.
Carter, Martine
Freeman, Kevin
Hallsworth, Peter
Limnios, E. Athena
Lum, Gary
Merien, Fabrice
Vernel-Pauillac, Frédérique
Tapsall, John W.
Witt, Michael J.
Nissen, Michael D.
Sloots, Theo P.
Title Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target
Journal name Pathology   Check publisher's open access policy
ISSN 0031-3025
Publication date 2006
Sub-type Article (original research)
DOI 10.1080/00313020600928253
Volume 38
Issue 5
Start page 445
End page 448
Total pages 4
Editor C. S. Lee
Place of publication Abingdon, UK
Publisher Taylor & Francis Ltd
Collection year 2006
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Formatted abstract
Aims:
The primary aim of the study was to determine if the gonococcal porA pseudogene is a stable sequence target for the detection of Neisseria gonorrhoeae by PCR.

Methods:
A total of 240 gonococcal strains from various geographic locations were tested by porA pseudogene PCR. In addition, porA pseudogene PCR positivity rates were compared with established gonococcal assays in three Australian states.

Results:
All N. gonorrhoeae isolates provided positive results in the porA pseudogene PCR. Positivity rates compared favourably with established gonococcal assays, with increased N. gonorrhoeae detection in the Northern Territory and Western Australia.

Conclusions:
The results of this multicentre study provide further evidence that the porA pseudogene is highly conserved across a diverse range N. gonorrhoeae strains and is a suitable PCR target for routine detection of N. gonorrhoeae.

Keyword Pathology
Neisseria
Gonorrhoeae
Pcr
Pora Pseudogene
Time Pcr Assay
Cppb Gene
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Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 08:44:00 EST