Detection and discrimination of herpes simplex viruses, Haemophilus ducreyi, Treponema pallidum, and Calymmatobacterium (Klebsiella) granulomatis from genital ulcers

Mackay, Ian M., Harnett, Gerry, Jeoffreys, Neisha, Bastian, Ivan, Sriprakash, Kadaba S., Siebert, David and Sloots, Theo P. (2006) Detection and discrimination of herpes simplex viruses, Haemophilus ducreyi, Treponema pallidum, and Calymmatobacterium (Klebsiella) granulomatis from genital ulcers. Clinical Infectious Diseases, 42 10: 1431-1438. doi:10.1086/503424


Author Mackay, Ian M.
Harnett, Gerry
Jeoffreys, Neisha
Bastian, Ivan
Sriprakash, Kadaba S.
Siebert, David
Sloots, Theo P.
Title Detection and discrimination of herpes simplex viruses, Haemophilus ducreyi, Treponema pallidum, and Calymmatobacterium (Klebsiella) granulomatis from genital ulcers
Journal name Clinical Infectious Diseases   Check publisher's open access policy
ISSN 1058-4838
Publication date 2006
Sub-type Article (original research)
DOI 10.1086/503424
Volume 42
Issue 10
Start page 1431
End page 1438
Total pages 8
Editor Sherwood L Gorbach
Place of publication Chicago
Publisher University Chicago Press
Collection year 2006
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract Background. Genital ulcer disease (GUD) is commonly caused by pathogens for which suitable therapies exist, but clinical and laboratory diagnoses may be problematic. This collaborative project was undertaken to address the need for a rapid, economical, and sensitive approach to the detection and diagnosis of GUD using noninvasive techniques to sample genital ulcers. Methods. The genital ulcer disease multiplex polymerase chain reaction (GUMP) was developed as an inhouse nucleic acid amplification technique targeting serious causes of GUD, namely, herpes simplex viruses (HSVs), Haemophilus ducreyi, Treponema pallidum, and Klebsiella species. In addition, the GUMP assay included an endogenous internal control. Amplification products from GUMP were detected by enzyme linked amplicon hybridization assay (ELAHA). Results. GUMP-ELAHA was sensitive and specific in detecting a target microbe in 34.3% of specimens, including 1 detection of HSV-1, three detections of HSV-2, and 18 detections of T. pallidum. No H. ducreyi has been detected in Australia since 1998, and none was detected here. No Calymmatobacterium ( Klebsiella) granulomatis was detected in the study, but there were 3 detections during ongoing diagnostic use of GUMP-ELAHA in 2004 and 2005. The presence of C. granulomatis was confirmed by restriction enzyme digestion and nucleotide sequencing of the 16S rRNA gene for phylogenetic analysis. Conclusions. GUMP-ELAHA permitted comprehensive detection of common and rare causes of GUD and incorporated noninvasive sampling techniques. Data obtained by using GUMP-ELAHA will aid specific treatment of GUD and better define the prevalence of each microbe among at-risk populations with a view to the eradication of chancroid and donovanosis in Australia.
Keyword Genital Ulcers
Haemophilus Ducreyi
Treponema Pallidum
Calymmatobacterium (klebsiella) Granulomatis
Gump-elaha
Immunology
Infectious Diseases
Microbiology
Polymerase-chain-reaction
Pcr Assay
Chlamydia-pneumoniae
Respiratory Samples
Molecular Methods
Donovanosis
Disease
Infection
Diagnosis
Quantification
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 08:36:24 EST