Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus

Dietzgen, RG, Callaghan, B, Wetzel, T and Dale, JL (2006) Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus. Virus Research, 118 -: 16-22. doi:10.1016/j.virusres.2005.10.024


Author Dietzgen, RG
Callaghan, B
Wetzel, T
Dale, JL
Title Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus
Journal name Virus Research   Check publisher's open access policy
ISSN 0168-1702
Publication date 2006
Sub-type Article (original research)
DOI 10.1016/j.virusres.2005.10.024
Open Access Status
Volume 118
Issue -
Start page 16
End page 22
Total pages 7
Place of publication Amsterdam
Publisher Elsevier Science
Collection year 2006
Language eng
Subject C1
270303 Virology
620208 Vegetables
Abstract We completed the genome sequence of Lettuce necrotic yellows virus (LNYV) by determining the nucleotide sequences of the 4a (putative phosphoprotein), 4b, M (matrix protein), G (glycoprotein) and L (polymerase) genes. The genome consists of 12,807 nucleotides and encodes six genes in the order 3' leader-N-4a(P)-4b-M-G-L-5' trailer. Sequences were derived from clones of a cDNA library from LNYV genomic RNA and from fragments amplified using reverse transcription-polymerase chain reaction. The 4a protein has a low isoelectric point characteristic for rhabdovirus phosphoproteins. The 4b protein has significant sequence similarities with the movement proteins of capillo- and trichoviruses and may be involved in cell-to-cell movement. The putative G protein sequence contains a predicted 25 amino acids signal peptide and endopeptidase cleavage site, three predicted glycosylation sites and a putative transmembrane domain. The deduced L protein sequence shows similarities with the L proteins of other plant rhabdoviruses and contains polymerase module motifs characteristic for RNA-dependent RNA polymerases of negative-strand RNA viruses. Phylogenetic analysis of this motif among rhabdoviruses placed LNYV in a group with other sequenced cytorhabdoviruses, most closely related to Strawberry crinkle virus. (c) 2005 Elsevier B.V. All rights reserved.
Keyword Plant Rhabdovirus
Lnyv
Phylogeny
Virology
Vesicular Stomatitis-virus
Polyadenylated Rnas
Stunt Rhabdovirus
Nucleocapsid Gene
Net Virus
Identification
Proteins
Plant
Organization
Variability
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 08:33:08 EST