Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine

Chapman, Toni A., Wu, Xi-Yang, Barchia, Idris, Bettelheim, Karl A., Driesen, Steven, Trott, Darren, Wilson,Mark and Chin, James J. C. (2006) Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine. Applied and Environmental Microbiology, 72 7: 4782-4795. doi:10.1128/AEM.02885-05

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Author Chapman, Toni A.
Wu, Xi-Yang
Barchia, Idris
Bettelheim, Karl A.
Driesen, Steven
Trott, Darren
Wilson,Mark
Chin, James J. C.
Title Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine
Formatted title
Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2006
Sub-type Article (original research)
DOI 10.1128/AEM.02885-05
Open Access Status File (Publisher version)
Volume 72
Issue 7
Start page 4782
End page 4795
Total pages 14
Editor L. Nicholson Ornston
Place of publication Washington DC, United States
Publisher American Society for Microbiology
Collection year 2006
Language eng
Subject C1
300507 Microbiology (excl. Virology)
300501 Veterinary Medicine
Abstract A combination of uni- and multiplex PCR assays targeting 58 virulence genes (VGs) associated with Escherichia coli strains causing intestinal and extraintestinal disease in humans and other mammals was used to analyze the VG repertoire of 23 commensal E. coli isolates from healthy pigs and 52 clinical isolates associated with porcine neonatal diarrhea (ND) and postweaning diarrhea (PWD). The relationship between the presence and absence of VGs was interrogated using three statistical methods. According to the generalized linear model, 17 of 58 VGs were found to be significant (P < 0.05) in distinguishing between commensal and clinical isolates. Nine of the 17 genes represented by iha, hlyA, aidA, east1, aah, fimH, iroN(E).(coli), traT, and saa have not been previously identified as important VGs in clinical porcine isolates in Australia. The remaining eight VGs code for fimbriae (F4, F5, F18, and F41) and toxins (STa, STh, LT, and Stx2), normally associated with porcine enterotoxigenic E. coli. Agglomerative hierarchical algorithm analysis grouped E. coli strains into subclusters based primarily on their serogroup. Multivariate analyses of clonal relationships based on the 17 VGs were collapsed into two-dimensional space by principal coordinate analysis. PWD clones were distributed in two quadrants, separated from ND and commensal clones, which tended to cluster within one quadrant. Clonal subclusters within quadrants were highly correlated with serogroups. These methods of analysis provide different perspectives in our attempts to understand how commensal and clinical porcine enterotoxigenic E. coli strains have evolved and are engaged in the dynamic process of losing or acquiring VGs within the pig population.
Keyword Virulence
Gene
Profile
Escherichia coli
Multiplex Pcr
Aida-i
Gastrointestinal-tract
Postweaning diarrhea
Fimbrial antigens
Diffuse adherence
Pigs
Toxin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Supplemental material (read only) - http://aem.asm.org/content/72/7/4782/suppl/DC1

 
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Created: Wed, 15 Aug 2007, 08:32:56 EST