Comparison of selected methods for measuring the virulence properties of Listeria spp.

Chiu, S, Vanderlinde, PB and Dykes, GA (2006) Comparison of selected methods for measuring the virulence properties of Listeria spp.. Canadian Journal of Microbiology, 52 4: 301-307. doi:10.1139/W05-129

Author Chiu, S
Vanderlinde, PB
Dykes, GA
Title Comparison of selected methods for measuring the virulence properties of Listeria spp.
Journal name Canadian Journal of Microbiology   Check publisher's open access policy
ISSN 0008-4166
Publication date 2006
Sub-type Article (original research)
DOI 10.1139/W05-129
Volume 52
Issue 4
Start page 301
End page 307
Total pages 7
Place of publication Ottawa
Publisher Natl Research Council Canada
Collection year 2006
Language eng
Subject C1
270301 Bacteriology
730216 Food safety
Abstract The comparative ability of different methods to assess virulence of Listeria species was investigated in ten Listeria strains. All strains were initially subjected to pulsed-field gel electrophoresis analysis to determine their relatedness. Virulence characteristics were subsequently tested for by (i) determining the presence of six virulence genes by polymerase chain reaction; (ii) testing for the production of listeriolysin O, phosphatidylcholine phospholipase C, and phosphatidylinositol-specific phospholipase C; (iii) investigating the hydrophobicity of the strains; (iv) determining the strains ability to attach to, enter, and replicate within the Caco-2 cells. Variations in most of the virulence characteristics were obvious across the strains for the range of tests performed. A wide range of anomalous results among methods were apparent. In particular, the presence of virulence genes was found to be unrelated to the production of virulence-associated proteins in vitro, while virulence protein production and hydrophobicity in Listeria monocytogenes were found to be unrelated or marginally related, respectively, to the ability to invade the Caco-2 cell line. It was concluded that the methods investigated were unable to consistently and unequivocally measure the differences in the virulence properties of the strains.
Keyword Listeria Monocytogenes
Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Field Gel-electrophoresis
Cell-surface Hydrophobicity
Monocytogenes Strains
Febrile Gastroenteritis
Nonpathogenic Listeria
Caco-2 Cell
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2007 Higher Education Research Data Collection
School of Agriculture and Food Sciences
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 08:32:49 EST