Archaeal diversity in two thermophilic chalcopyrite bioleaching reactors

Mikkelsen, Deirdre, Kappler, Ulrike, McEwan, Alastair G. and Sly, Lindsay I. (2006) Archaeal diversity in two thermophilic chalcopyrite bioleaching reactors. Environmental Microbiology, 8 11: 2050-2055. doi:10.1111/j.1462-2920.2005.01115.x

Author Mikkelsen, Deirdre
Kappler, Ulrike
McEwan, Alastair G.
Sly, Lindsay I.
Title Archaeal diversity in two thermophilic chalcopyrite bioleaching reactors
Journal name Environmental Microbiology   Check publisher's open access policy
ISSN 1462-2912
Publication date 2006-11
Sub-type Article (original research)
DOI 10.1111/j.1462-2920.2005.01115.x
Volume 8
Issue 11
Start page 2050
End page 2055
Total pages 6
Place of publication Oxford
Publisher Blackwell Publishing
Collection year 2006
Language eng
Subject C1
270307 Microbial Ecology
771099 Other
Abstract This study used a culture-independent molecular approach to investigate the archaeal community composition of thermophilic bioleaching reactors. Two culture samples, MTC-A and MTC-B, grown with different concentrations of chalcopyrite (CuFeS2), a copper sulfidic ore, at a temperature of 78 degrees C and pH 1.6 were studied. Phylogenetic analysis of the 16S rRNA genes revealed that both cultures consisted of Archaea belonging to the Sulfolobales. The 16S rRNA gene clone library of MTC-A grown with 4% (w/v) chalcopyrite was dominated by a unique phylotype related to Sulfolobus shibatae (69% of total clones). The remaining clones were affiliated with Stygiolobus azoricus (11%), Metallosphaera sp. J1 (8%), Acidianus infernus (2%), and a novel phylotype related to Sulfurisphaera ohwakuensis (10%). In contrast, the clones from MTC-B grown with 12% (w/v) chalcopyrite did not appear to contain Sulfolobus shibatae-like organisms. Instead the bioleaching consortium was dominated by clones related to Sulfurisphaera ohwakuensis (73.9% of total clones). The remaining microorganisms detected were similar to those found in MTC-A.
Keyword Microbiology
Acidophilic Microorganisms
Q-Index Code C1
Additional Notes DOI: 10.1111/j.1462-2920.2006.01115.x

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Created: Wed, 15 Aug 2007, 08:26:25 EST