Archaeal diversity in two thermophilic chalcopyrite bioleaching reactors

Mikkelsen, Deirdre, Kappler, Ulrike, McEwan, Alastair G. and Sly, Lindsay I. (2006) Archaeal diversity in two thermophilic chalcopyrite bioleaching reactors. Environmental Microbiology, 8 11: 2050-2055.


Author Mikkelsen, Deirdre
Kappler, Ulrike
McEwan, Alastair G.
Sly, Lindsay I.
Title Archaeal diversity in two thermophilic chalcopyrite bioleaching reactors
Journal name Environmental Microbiology  (ERA 2012 Listed)    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2006-11
Sub-type Article
DOI 10.1111/j.1462-2920.2005.01115.x
Volume number 8
Issue number 11
ISSN 1462-2912
Start page 2050
End page 2055
Total pages 6
Place of publication Oxford
Publisher Blackwell Publishing
Collection year 2006
Language eng
Subject C1
270307 Microbial Ecology
771099 Other
Abstract This study used a culture-independent molecular approach to investigate the archaeal community composition of thermophilic bioleaching reactors. Two culture samples, MTC-A and MTC-B, grown with different concentrations of chalcopyrite (CuFeS2), a copper sulfidic ore, at a temperature of 78 degrees C and pH 1.6 were studied. Phylogenetic analysis of the 16S rRNA genes revealed that both cultures consisted of Archaea belonging to the Sulfolobales. The 16S rRNA gene clone library of MTC-A grown with 4% (w/v) chalcopyrite was dominated by a unique phylotype related to Sulfolobus shibatae (69% of total clones). The remaining clones were affiliated with Stygiolobus azoricus (11%), Metallosphaera sp. J1 (8%), Acidianus infernus (2%), and a novel phylotype related to Sulfurisphaera ohwakuensis (10%). In contrast, the clones from MTC-B grown with 12% (w/v) chalcopyrite did not appear to contain Sulfolobus shibatae-like organisms. Instead the bioleaching consortium was dominated by clones related to Sulfurisphaera ohwakuensis (73.9% of total clones). The remaining microorganisms detected were similar to those found in MTC-A.
Keyword Microbiology
Cell-wall-lacking
Acidophilic Microorganisms
Sp-nov
Biooxidation
Bacteria
Leptospirillum
Biodiversity
Ferrooxidans
Ferroplasma
Q-Index Code C1
Additional Notes DOI: 10.1111/j.1462-2920.2006.01115.x

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 134 Abstract Views  -  Detailed Statistics
Created: Wed, 15 Aug 2007, 08:26:25 EST