Antioxidant supplementation enhances erythrocyte antioxidant status and attenuates cyclosporine-induced vascular dysfunction

Lexis, L. A., Fenning, A., Brown, L., Fassett, R. G. and Coombes, J. S. (2006) Antioxidant supplementation enhances erythrocyte antioxidant status and attenuates cyclosporine-induced vascular dysfunction. American Journal of Transplantation, 6 1: 41-49. doi:10.1111/j.1600-6143.2005.01154.x


Author Lexis, L. A.
Fenning, A.
Brown, L.
Fassett, R. G.
Coombes, J. S.
Title Antioxidant supplementation enhances erythrocyte antioxidant status and attenuates cyclosporine-induced vascular dysfunction
Journal name American Journal of Transplantation   Check publisher's open access policy
ISSN 1600-6135
Publication date 2006
Sub-type Article (original research)
DOI 10.1111/j.1600-6143.2005.01154.x
Volume 6
Issue 1
Start page 41
End page 49
Total pages 9
Editor P.F. Halloran
Place of publication Oxford
Publisher Blackwell Publishing
Collection year 2006
Language eng
Subject C1
321003 Cardiology (incl. Cardiovascular Diseases)
730106 Cardiovascular system and diseases
Abstract The aim of this study was to determine the effects of dietary antioxidant supplementation with a-tocopherol and a-lipoic acid on cyclosporine-induced alterations to erythrocyte and plasma redox balance, and cyclosporine-induced endothelial and smooth muscle dysfunction. Rats were randomly assigned to either control, antioxidant, cyclosporine or cyclosporine + antioxidant treatments. Cyclosporine A was administered for 10 days after an 8-week feeding period. Plasma was analyzed for alpha-tocopherol, total antioxidant capacity, malondialdehyde and creatinine. Erythrocytes were analyzed for glutathione, methemoglobin, superoxide dismutase, catalase, glutathione peroxidase, glucose-6-phosphate dehydrogenase, alpha-tocopherol and malondialdehye. Vascular endothelial and smooth muscle function was determined in vitro. Antioxidant supplementation resulted in significant increases in erythrocyte a-tocopherol concentration and glutathione peroxidase activity in both of the antioxidant-supplemented groups. Cyclosporine administration caused significant decreases in glutathione concentration, methemoglobin concentration and superoxide dismutase activity. Antioxidant supplementation attenuated the cyclosporine-induced decrease in superoxide dismutase activity. Cyclosporine therapy impaired both endothelium-independent and -dependent relaxation of the thoracic aorta, and this was attenuated by antioxidant supplementation. In summary, dietary supplementation with alpha-tocopherol and alpha-lipoic acid attenuated the cyclosporine-induced decrease in erythrocyte superoxide dismutase activity and attenuated cyclosporine-induced vascular dysfunction.
Keyword Antioxidant Status
Cyclosporine A
Erythrocytes
Oxidative Stress
Vascular Function
Surgery
Transplantation
Alpha-lipoic Acid
Induced Hypertensive Rats
Coronary-artery-disease
A-induced Changes
Vitamin-e
Nitric-oxide
Endothelial Dysfunction
Lipid-peroxidation
Transplant Recipients
Q-Index Code C1

 
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