Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source

Oehmen, A, Zeng, RJ, Saunders, AM, Blackall, LL, Keller, J and Yuan, ZG (2006) Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source. Microbiology-sgm, 152 Part 9: 2767-2778.


Author Oehmen, A
Zeng, RJ
Saunders, AM
Blackall, LL
Keller, J
Yuan, ZG
Title Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source
Journal name Microbiology-sgm  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2006
Sub-type Article
DOI 10.1099/mic.0.28065-0
Volume number 152
Issue number Part 9
ISSN 1350-0872
Start page 2767
End page 2778
Total pages 12
Editor C.J. Dorman
Place of publication Reading
Publisher Soc General Microbiology
Collection year 2006
Language eng
Subject C1
270399 Microbiology not elsewhere classified
779999 Other
Abstract In the microbial competition observed in enhanced biological phosphorus removal (EBPR) systems, an undesirable group of micro-organisms known as glycogen-accumulating organisms (GAOs) compete for carbon in the anaerobic period with the desired polyphosphate-accumulating organisms (PAOs). Some studies have suggested that a propionate carbon source provides PAOs with a competitive advantage over GAOs in EBPR systems; however, the metabolism of GAOs with this carbon source has not been previously investigated. In this study, GAOs were enriched in a laboratory-scale bioreactor with propionate as the sole carbon source, in an effort to better understand their biochemical processes. Based on comprehensive solid-, liquid- and gas-phase chemical analytical data from the bioreactor, a metabolic model was proposed for the metabolism of propionate by GAOs. The model adequately described the anaerobic stoichiometry observed through chemical analysis, and can be a valuable tool for further investigation of the competition between PAOs and GAOs, and for the optimization of the EBPR process. A group of Alphaproteobacteria dominated the biomass (96% of Bacteria) from this bioreactor, while post-fluorescence in situ hybridization (FISH) chemical staining confirmed that these Alphaproteobacteria produced poly-beta-hydroxyalkanoates (PHAs) anaerobically and utilized them aerobically, demonstrating that they were putative GAOs. Some of the Alphaproteobacteria were related to Defluvicoccus vanus (16% of Bacteria), but the specific identity of many could not be determined by FISH. Further investigation into the identity of other GAOs is necessary.
Keyword -anaerobic And Aerobic Metabolism
Carbon Source
Microbiology
Biological Phosphorus Removal
Activated-sludge Systems
In-situ Identification
Laboratory-scale
Microbial-populations
Acetate Uptake
Polyphosphate
Model
Stoichiometry
Competition
Q-Index Code C1

 
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