An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination

Tobe, Toru, Beatson, Scott A., Taniguchi, Hisaaki, Abe, Hiroyuki, Bailey, Christopher M., Fivian, Amanda, Younis, Rasha, Matthews, Sophie, Marches, Olivier, Frankel, Gad, Hayashi, Tetsuya and Pallen, Mark J. (2006) An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination. Proceedings of The National Academy of Sciences of The United States of America, 103 40: 14941-14946.


Author Tobe, Toru
Beatson, Scott A.
Taniguchi, Hisaaki
Abe, Hiroyuki
Bailey, Christopher M.
Fivian, Amanda
Younis, Rasha
Matthews, Sophie
Marches, Olivier
Frankel, Gad
Hayashi, Tetsuya
Pallen, Mark J.
Title An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination
Journal name Proceedings of The National Academy of Sciences of The United States of America   Check publisher's open access policy
ISSN 0027-8424
Publication date 2006
Sub-type Article (original research)
DOI 10.1073/pnas.0604891103
Volume 103
Issue 40
Start page 14941
End page 14946
Total pages 6
Place of publication Washington
Publisher Natl Acad Sciences
Collection year 2006
Language eng
Subject C1
270199 Biochemistry and Cell Biology not elsewhere classified
780105 Biological sciences
1108 Medical Microbiology
060408 Genomics
Abstract Several pathogenic strains of Escherichia coli exploit type III secretion to inject effector proteins into human cells, which then subvert eukaryotic cell biology to the bacterium's advantage. We have exploited bioinformatics and experimental approaches to establish that the effector repertoire in the Sakai strain of enterohemorrhagic E. coli (EHEC) O157:H7 is much larger than previously thought. Homology searches led to the identification of > 60 putative effector genes. Thirteen of these were judged to be likely pseudogenes, whereas 49 were judged to be potentially functional. In total, 39 proteins were confirmed experimentally as effectors: 31 through proteomics and 28 through translocation assays. At the protein level, the EHEC effector sequences fall into > 20 families. The largest family, the NleG family, contains 14 members in the Sakai strain alone. EHEC also harbors functional homologs of effectors from plant pathogens (HopPtoH, HopW, AvrA) and from Shigella (OspD, OspE, OspG), and two additional members of the Map/IpgB family. Genes encoding proven or predicted effectors occur in > 20 exchangeable effector loci scattered throughout the chromosome. Crucially, the majority of functional effector genes are encoded by nine exchangeable effector loci that lie within lambdoid prophages. Thus, type III secretion in E. coli is linked to a vast phage metagenome, acting as a crucible for the evolution of pathogenicity.
Keyword Bacterial Pathogenesis
Bacterial Protein Secretion
Bioinformatics
Genomics
Virulence
Multidisciplinary Sciences
Pathogen Pseudomonas-syringae
Shigella-flexneri
Salmonella-typhimurium
Genetic-structure
Acid-sequence
Hela-cells
Protein
System
Identification
Q-Index Code C1

 
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