Age determination in individual wild-caught Drosophila serrata using pteridine concentration

Robson, S. K. A., Vickers, M., Blows, M. W. and Crozier, R. H. (2006) Age determination in individual wild-caught Drosophila serrata using pteridine concentration. Journal of Experimental Biology, 209 16: 3155-3163.


Author Robson, S. K. A.
Vickers, M.
Blows, M. W.
Crozier, R. H.
Title Age determination in individual wild-caught Drosophila serrata using pteridine concentration
Journal name Journal of Experimental Biology  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2006
Sub-type Article
DOI 10.1242/jeb.02318
Volume number 209
Issue number 16
ISSN 0022-0949
Start page 3155
End page 3163
Total pages 9
Place of publication Cambridge
Publisher Company of Biologists Ltd.
Collection year 2006
Language eng
Subject C1
270706 Life Histories (incl. Population Ecology)
780105 Biological sciences
Abstract Fluorescence spectrophotometry can reliably detect levels of the pteridine 6-biopterin in the heads of individual Drosophila serrata Malloch 1927. Pteridine content in both laboratory and field captured flies is typically a level of magnitude higher than the minimally detectable level (mean(lab)=0.54 units, mean(field)=0.44 units, minimum detectable level=0.01 units) and can be used to predict individual age in laboratory populations with high certainty (r(2)=57%). Laboratory studies of individuals of known age ( from 1 to 48 days old) indicate that while pteridine level increases linearly with age, they also increase in a linear manner with rearing temperature and ambient light levels, but are independent of sex. As expected, the longevity of laboratory-reared males ( at least 48 days) is higher than the range of predicted ages of wild-caught males based on individual pteridine levels (40 days). However, the predictive equation based on pteridine level alone suggested that a number of wild-caught males were less than 0 days old, and the 95% confidence for these predictions based on the inverse regression broad. The age of the oldest wild-caught male is to fall within the range of 2 to 50 days. The effects of temperature and light intensity determined in laboratory study (effect sizes omega(2)=14.3 and respectively) suggests that the calibration of the prediction equation for field populations would significantly improved when combined with fine scaled studies of habitat temperature and light conditions. ability to determine relative age in individual wild-caught D. serrata presents great opportunities for a variety evolutionary studies on the dynamics of populations.
Keyword Age Determination
Pteridine
Drosophila Serrata
Survivor Function
Biology
Stomoxys-calcitrans
Flies Diptera
Tsetse-flies
Melanogaster
Fluorescence
Accumulation
Fly
Glossina
Muscidae
Tephritidae
Q-Index Code C1

 
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