AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars

Aitken, K. S., Li, J. C., Jackson, P., Piperidis, G. and McIntyre, C. L. (2006) AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars. Australian Journal of Agricultural Research, 57 11: 1167-1184. doi:10.1071/AR05391

Author Aitken, K. S.
Li, J. C.
Jackson, P.
Piperidis, G.
McIntyre, C. L.
Title AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars
Journal name Australian Journal of Agricultural Research   Check publisher's open access policy
ISSN 0004-9409
Publication date 2006
Sub-type Article (original research)
DOI 10.1071/AR05391
Volume 57
Issue 11
Start page 1167
End page 1184
Total pages 18
Place of publication Collingwood
Publisher CSIRO Publishing
Collection year 2006
Language eng
Subject C1
270202 Genome Structure
620106 Sugar
Abstract Molecular diversity among 421 clones of cultivated sugarcane and wild relatives was analysed using AFLP markers. Of these clones, 270 were Saccharum officinarum and 151 were either cultivars produced by the Australian breeding program or important parents used in the breeding program. The S. of. cinarum clones were obtained from a collection that contained clones from all the major regions where S. of. cinarum is grown. Five AFLP primer combinations generated 657 markers ofwhich 614 were polymorphic. All clones contained a large number of markers; a result of the polyploid nature and heterozygosity of the genome. S. of. cinarum clones from New Guinea displayed greater diversity than S. of. cinarum clones from other regions. This is in agreement with the hypothesis that New Guinea is the centre of origin of this species. The S. of. cinarum clones from Hawaii and Fiji formed a separate group and may correspond to clones that have been introgressed with other members of the ` Saccharum complex'. Greater diversity was found in the cultivars than in the S. of. cinarum clones due to the introgression of S. spontaneum chromatin. These cultivars clustered as expected based on pedigree. The major contribution of clones QN66- 2008 and Nco310 to Australian sugarcane cultivars divided the cultivars into 2 main groups. Although only a fewS. of. cinarum clones are known to have been used in the breeding of current cultivars, about 90% of markers present in the S. of. cinarum clone collection ( 2n= 80) were also present in the cultivar collection. This suggests that most of the observed genetic diversity in S. of. cinarum has been captured in Australian sugarcane germplasm.
Keyword Molecular Markers
Agriculture, Multidisciplinary
Genome Structure
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2007 Higher Education Research Data Collection
School of Agriculture and Food Sciences
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 08:23:57 EST