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Actin localisation and the effect of cytochalasin D on the osmotic tolerance of cauda epedidymidal kangaroo spermatozoa
McClean, R., MacCallum, C., Blyde, D., Hole, W. and Johnston, S. (2006-06) Actin localisation and the effect of cytochalasin D on the osmotic tolerance of cauda epedidymidal kangaroo spermatozoa. Cryoletters, 27 4: 253-260.
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| Author(s) |
McClean, R.
MacCallum, C.
Blyde, D.
Hole, W.
Johnston, S.
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| Title |
Actin localisation and the effect of cytochalasin D on the osmotic tolerance of cauda epedidymidal kangaroo spermatozoa
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| Journal name |
Cryoletters
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| Publication date |
2006-06
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| Volume number |
27
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| Issue number |
4
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| ISSN |
0143-2044
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| Start page |
253
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| End page |
260
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| Total pages |
8
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| Editor(s) |
P. F. Watson
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| Place of publication |
London
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| Publisher |
Cryo Letters
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| Collection year |
2006
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| Language |
eng
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| Subject |
C1
270602 Animal Physiology - Cell
780105 Biological sciences
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| Abstract |
This study examined the hypothesis that filamentous actin associated with the complex cytoskeleton of the kangaroo sperm head and tail may be contributing to lack of plasma membrane plasticity and a consequent loss of membrane integrity during cryopreservation. In the first study, the distribution of G and F actin within Eastern Grey Kangaroo (EGK, Macropus giganteus) cauda epididymidal spermatozoa was successfully detected using DNAse-FITC and a monoclonal F-actin antibody (ab205, Abcam), respectively. G-actin staining was most intense in the acrosome but was also observed with less intensity over the nucleus and mid-piece. F-actin was located in the sperm nucleus but was not discernable in the acrosome or sperm tail. To investigate whether cytochalasin D (a known F-actin depolymerising agent) was capable of improving the osmotic tolerance of EGK cauda epididymal spermatozoa, sperm were incubated in hypo-osmotic media (61 and 104 mOsm) containing a range of cytochalasin D concentrations (0-200 mu M). Cytochalasin D had no beneficial effect on plasma membrane integrity of sperm incubated in hypo-osmotic media. However, when EGK cauda epididymidal sperm were incubated in isosmotic media, there was a progressive loss of sperm motility with increasing cytochalasin D concentration. The results of this study indicated that the F-actin distribution in cauda epididymidal spermatozoa of the EGK was surprisingly different from that of the Tammar Wallaby (M. eugenii) and that cytochalasin-D does not appear to improve the tolerance of EGK cauda epididymidal sperm to osmotically induced injury.
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| Keyword(s) |
Biology
Physiology
kangaroo
spermatozoa
cryopreservation
osmotic tolerance
F-actin
cytochalasin D
Mammalian Spermatozoa
F-actin
Acrosome
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