A need for caution in the use of frontal analysis continuous capillary electrophoresis for the determination of ligand binding data

Winzor, D. J. (2006) A need for caution in the use of frontal analysis continuous capillary electrophoresis for the determination of ligand binding data. Analytical Biochemistry, 349 2: 285-291.


Author Winzor, D. J.
Title A need for caution in the use of frontal analysis continuous capillary electrophoresis for the determination of ligand binding data
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
Publication date 2006
Sub-type Article (original research)
DOI 10.1016/j.ab.2005.11.028
Volume 349
Issue 2
Start page 285
End page 291
Total pages 7
Place of publication San Diego
Publisher Academic Press Inc Elsevier Science
Collection year 2006
Language eng
Subject C1
270100 Biochemistry and Cell Biology
780105 Biological sciences
Abstract Attention is drawn to a need for caution in the determination of binding data for protein-polyelectrolyte interactions by frontal analysis continuous capillary electrophoresis (FACCE). Because the method is valid only for systems involving comigration of complex(es) and slower-migrating reactant, establishing conformity with that condition is clearly a prerequisite for its application. However, that requirement has not been tested in any published studies thus far. On the basis of calculated FACCE patterns, presented to illustrate features by which such comigration of complex(es) and slower-migrating reactant can be identified, the form of the published pattern for a P-lactoglobulin-poly(styrenesulfonate) mixture does not seem to signify the migration behavior required to justify its consideration in such terms. Additional experimental studies are therefore needed to ascertain the validity of FACCE as a means of determining binding data for the characterization of protein-polyelectrolyte interactions. (c) 2005 Elsevier Inc. All rights reserved.
Keyword Frontal Analysis Continuous Capillary Clectrophoresis
Protein-polyelectrolyte Interaction
Gilbert-jenkins Theory
Biochemical Research Methods
Biochemistry & Molecular Biology
Chemistry, Analytical
Reacting Systems
Serum-albumin
Constants
Polyelectrolytes
Dependence
Complexes
Q-Index Code C1

 
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