A method for screening the temperature dependence of three-dimensional crystal formation

Landsberg, M. J., Bond, J., Gee, C. L., Martin, J. L. and Hankamer, B. (2006) A method for screening the temperature dependence of three-dimensional crystal formation. Acta Crystallographica Section D-biological Crystallography, 62 559-562. doi:10.1107/s0907444906006093


Author Landsberg, M. J.
Bond, J.
Gee, C. L.
Martin, J. L.
Hankamer, B.
Title A method for screening the temperature dependence of three-dimensional crystal formation
Journal name Acta Crystallographica Section D-biological Crystallography   Check publisher's open access policy
ISSN 0907-4449
Publication date 2006
Sub-type Article (original research)
DOI 10.1107/s0907444906006093
Volume 62
Start page 559
End page 562
Total pages 4
Editor E N Baker
Z Dauter
Place of publication Oxford
Publisher Blackwell Publishing
Collection year 2006
Language eng
Subject C1
270802 Diagnostic Applications
671401 Scientific instrumentation
Abstract Temperature is an important parameter controlling protein crystal growth. A new temperature-screening system (Thermo-screen) is described consisting of a gradient thermocycler fitted with a special crystallization-plate adapter onto which a 192-well sitting-drop crystallization plate can be mounted (temperature range 277-372 K; maximum temperature gradient 20 K; interval precision 0.3 K). The system allows 16 different conditions to be monitored simultaneously over a range of 12 temperatures and is well suited to conduct wide (similar to 20 K) and fine (similar to 3 K) temperature-optimization screens. It can potentially aid in the determination of temperature phase diagrams and run more complex temperature-cycling experiments for seeding and crystal growth.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Biophysics
Crystallography
Protein Crystallization
Aqueous-solution
Phase-diagrams
X-ray
Growth
Glucagon
Q-Index Code C1

 
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