A standardized method for analysis of Medicago truncatula phenotypic development

Bucciarelli, Bruna, Hanan, Jim, Palmquist, Debra and Vance, Carroll P. (2006) A standardized method for analysis of Medicago truncatula phenotypic development. Plant Physiology, 142 1: 207-219.


Author Bucciarelli, Bruna
Hanan, Jim
Palmquist, Debra
Vance, Carroll P.
Title A standardized method for analysis of Medicago truncatula phenotypic development
Formatted title A standardized method for analysis of Medicago truncatula phenotypic development
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
1532-2548
Publication date 2006-09
Sub-type Article (original research)
DOI 10.1104/pp.106.082594
Volume 142
Issue 1
Start page 207
End page 219
Total pages 13
Editor Donald R. Ort
John Long
Place of publication Rockville, MD, U.S.A.
Publisher American Society of Plant Biologists
Collection year 2006
Language eng
Subject C1
270402 Plant Physiology
620108 Grain legumes
0607 Plant Biology
Formatted abstract Medicago truncatula has become a model system to study legume biology. It is imperative that detailed growth characteristics of the most commonly used cultivar, line A17 cv Jemalong, be documented. Such analysis creates a basis to analyze phenotypic alterations due to genetic lesions or environmental stress and is essential to characterize gene function and its relationship to morphological development. We have documented morphological development of M. truncatula to characterize its temporal developmental growth pattern; developed a numerical nomenclature coding system that identifies stages in morphological development; tested the coding system to identify phenotypic differences under phosphorus (P) and nitrogen (N) deprivation; and created visual models using the L-system formalism. The numerical nomenclature coding system, based on a series of defined growth units, represents incremental steps in morphological development. Included is a decimal component dividing growth units into nine substages. A measurement component helps distinguish alterations that may be missed by the coding system. Growth under N and P deprivation produced morphological alterations that were distinguishable using the coding system and its measurement component. N and P deprivation resulted in delayed leaf development and expansion, delayed axillary shoot emergence and elongation, decreased leaf and shoot size, and altered root growth. Timing and frequency of flower emergence in P-deprived plants was affected. This numerical coding system may be used as a standardized method to analyze phenotypic variation in M. truncatula due to nutrient stress, genetic lesions, or other factors and should allow valid growth comparisons across geographically distant laboratories.
© 2006 American Society of Plant Biologists.
Keyword Plant sciences
Phosphorus-nutrition
Functional genomics
Growth-stages
Decimal code
Plants
Arabidopsis
Model
Availability
Architecture
Acquisition
Q-Index Code C1
Additional Notes DOI: 10.1104/pp.106.082594

 
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