A side order of stem cells: The SP phenotype

Challen, Grant A. and Little, Melissa H. (2006) A side order of stem cells: The SP phenotype. Stem Cells, 24 1: 3-12. doi:10.1634/stemcells.2005.0116

Author Challen, Grant A.
Little, Melissa H.
Title A side order of stem cells: The SP phenotype
Journal name Stem Cells   Check publisher's open access policy
ISSN 1066-5099
Publication date 2006-01
Sub-type Article (original research)
DOI 10.1634/stemcells.2005.0116
Volume 24
Issue 1
Start page 3
End page 12
Total pages 10
Editor Curt I Civin
Place of publication Durham
Publisher Alphamed Press
Collection year 2006
Language eng
Subject C1
270106 Cell Development (incl. Cell Division and Apoptosis)
780105 Biological sciences
Abstract A defining property of murine hematopoietic stein cells (HSCs) is low fluorescence after staining with Hoechst 33342 and Rhodamine 123. These dyes have proven to be remarkably powerful tools in the purification and characterization of HSCs when used alone or in combination with antibodies directed against stem cell epitopes. Hoechst low cells are described as side population (SP) cells by virtue of their typical profiles in Hoechst red versus Hoechst blue bivariate fluorescent-activated cell sorting dot plots. Recently, excitement has been generated by the findings that putative stem cells from solid tissues may also possess this SP phenotype. SP cells have now been isolated from a wide variety of mammalian tissues based on this same dye efflux phenomenon, and in many cases this cell population has been shown to contain apparently multipotent stem cells. What is yet to be clearly addressed is whether cell fusion accounts for this perceived SP multipotency. Indeed, if low fluorescence after Hoechst staining is a phenotype shared by hematopoietic and organ-specific stem cells, do all resident tissue SP cells have bone marrow origins or might the SP phenotype be a property common to all stem cells? Subject to further analysis, the SP phenotype may prove invaluable for the initial isolation of resident tissue stem cells in the absence of definitive cell-surface markers and may have broad-ranging applications in stem cell biology, from the purification of novel stem cell populations to the development of autologous stem cell therapies.
Keyword Side Population
Hoechst 33342
Stem Cell
Dye Efflux
Biotechnology & Applied Microbiology
Cell Biology
Population Cells
Progenitor Cells
Neural Stem
Q-Index Code C1

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 301 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 351 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 08:18:56 EST