A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter

Vickers, C. E., Xue, G. P. and Gresshoff, P. M. (2006) A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter. Plant Molecular Biology, 62 1-2: 195-214.


Author Vickers, C. E.
Xue, G. P.
Gresshoff, P. M.
Title A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
Publication date 2006
Sub-type Article (original research)
DOI 10.1007/s11103-006-9014-1
Volume 62
Issue 1-2
Start page 195
End page 214
Total pages 20
Place of publication Dordrecht
Publisher Springer
Collection year 2006
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
620108 Grain legumes
Abstract To examine the genetic controls of endosperm (ES) specificity, several cereal seed storage protein (SSP) promoters were isolated and studied using a transient expression analysis system. An oat globulin promoter (AsGlo1) capable of driving strong ES-specific expression in barley and wheat was identified. Progressive 5' deletions and cis element mutations demonstrated that the mechanism of specificity in the AsGlo1 promoter was distinct from that observed in glutelin and prolamin promoters. A novel interrupted palindromic sequence, ACATGTCAT-CATGT, was required for ES specificity and substantially contributed to expression strength of the AsGlo1 promoter. This sequence was termed the endosperm specificity palindrome (ESP) element. The GCN4 element, which has previously been shown to be required for ES specificity in cereal SSP promoters, had a quantitative role but was not required for tissue specificity. The 960-bp AsGlo1 promoter and a 251-bp deletion containing the ESP element also drove ES-specific expression in stably transformed barley. Reporter gene protein accumulated at very high levels (10% of total soluble protein) in ES tissues of plants transformed with an AsGlo1:GFP construct. Expression strength and tissue specificity were maintained over five transgenic generations. These attributes make the AsGlo1 promoter an ideal promoter for biotechnology applications. In conjunction with previous findings, our data demonstrate that there is more than one genetically distinct mechanism by which ES specificity can be achieved in cereal SSP promoters, and also suggest that there is redundancy between transcriptional and post-transcriptional tissue specificity mechanisms in cereal globulin genes.
Keyword Asglo1 Promoter
Esp Element
Endosperm Specificity
Gcn4 Box
Globulin Promoter
Promoter Analysis
Biochemistry & Molecular Biology
Plant Sciences
Tissue-specific Expression
Storage Protein Genes
Bzip Transcriptional Activator
Molecular-weight Glutenin
Green Fluorescent Protein
Soybean Beta-conglycinin
Rice Glutelin Gene
Transgenic Barley
Dna-binding
Bifactorial Endosperm
Q-Index Code C1

 
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